{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:31:36Z","timestamp":1762864296118},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":692,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> Calcification is a frequent cause of the clinical failure of bioprosthetic heart valves fabricated from glutaraldehyde pretreated bovine pericardium (GATBP). Aspirin, a potent antiplatelet drug, and heparin, an anticoagulant, are commonly used for postimplant complications such as thrombosis and thromboembolism. Aspirin and heparin were embedded in chitosan\/polyethylene vinylacetate co\u2010matrix to develop a prolonged release form. The effect of these drugs towards the bioprosthetic calcification was investigated by in vitro and in vivo models. In vitro and in vivo evaluation suggest that the released aspirin\/heparin from the co\u2010matrix had a synergistic effect in inhibiting GATBP calcification. In vivo subcutaneous co\u2010implantation was performed with PEG\u201020,000 grafted bovine pericardium (PEG\u2010GABP), aspirin, and heparin. Biochemical, histological, and scanning electron microscopic evaluation of retrieved samples demonstrated a significant reduction in calcium deposition and alkaline phosphatase activity on PEG\u2010GABP compared to GATBP. It seems that the aspirin\/heparin combination synergistically inhibits the pericardial calcification in addition to their antithrombotic function.<\/jats:p>","DOI":"10.1046\/j.1525-1594.2000.06348.x","type":"journal-article","created":{"date-parts":[[2003,3,10]],"date-time":"2003-03-10T22:10:18Z","timestamp":1047334218000},"page":"129-136","source":"Crossref","is-referenced-by-count":10,"title":["Synergistic Effect of Released Aspirin\/Heparin for Preventing Bovine Pericardial\u2003Calcification"],"prefix":"10.1111","volume":"24","author":[{"given":"Sindhu C.","family":"Vasudev","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Chandy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandra P.","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mira","family":"Mohanty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.R.","family":"Umasankar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0733-8651(18)30720-3","article-title":"Bioprosthetic heart valve failure pathology and pathogenesis.","volume":"2","author":"Schoen FJ","year":"1984","journal-title":"Cardiol Clin"},{"key":"e_1_2_7_3_2","first-page":"523","article-title":"Onset and progression of experimental bioprosthetic heart valve calcification.","volume":"52","author":"Schoen FJ","year":"1985","journal-title":"Lab Invest"},{"key":"e_1_2_7_4_2","first-page":"143","article-title":"Biologic determinants of dystrophic calcification and osteocalcin deposition in glutaraldehyde preserved porcine aortic valve leaflets implanted subcutaneously in rats.","volume":"113","author":"Levy RJ","year":"1983","journal-title":"Am J Pathol"},{"key":"e_1_2_7_5_2","first-page":"418","volume-title":"Biological and Bioprosthetic valves","author":"Schoen FJ","year":"1986"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(80)90421-X"},{"key":"e_1_2_7_7_2","first-page":"714","article-title":"Mineralization of artificial surfaces that contact blood.","volume":"27","author":"Nos\u00e9 Y","year":"1981","journal-title":"Trans Am Soc Artif Intern Organs"},{"key":"e_1_2_7_8_2","first-page":"702","article-title":"Normal and abnormal mineralization in mammals.","volume":"27","author":"Anderson HC","year":"1981","journal-title":"Trans Am Soc Artif Intern Organs"},{"key":"e_1_2_7_9_2","first-page":"215","article-title":"Role of membranes in calcification.","volume":"2","author":"Kim KM","year":"1983","journal-title":"Surv Synth Pathol Res"},{"key":"e_1_2_7_10_2","first-page":"11","article-title":"Biomaterials associated calcification. Pathology, mechanism, and strategies for prevention.","volume":"22","author":"Sochen JF","year":"1988","journal-title":"J Biomed Mater Res"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820250802"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(90)90034-N"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-3659(90)90137-I"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jab.770050310"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(96)88708-4"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(83)90357-2"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(83)90063-7"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(96)00131-7"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19970605)35:3<357::AID-JBM10>3.0.CO;2-C"},{"key":"e_1_2_7_20_2","first-page":"272","article-title":"Bovine pericardium for heart valve bioprostheses in vitro and in vivo characterization of new chemical treatments.","volume":"3","author":"Pasquino E","year":"1992","journal-title":"J Mater Sci Mater Med"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820280602"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90679-X"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(91)90008-X"},{"key":"e_1_2_7_24_2","first-page":"480","volume-title":"Practical biochemistry","author":"Varley H","year":"1967"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1177\/088532829901400103"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.1996.tb04536.x"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0267-6605(94)90032-9"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105903"},{"key":"e_1_2_7_29_2","first-page":"266","article-title":"Proteins, plasma, and blood in narrow spaces of clot promoting surfaces.","volume":"199","author":"Vroman L","year":"1982","journal-title":"Adv Chem"},{"key":"e_1_2_7_30_2","unstructured":"Vyavahare NR Fuller A Levy RJ. Controlled release of heparin from polymers. Effect on calcification of porcine aortic cusps.Proceedings of the 5th World Biomaterials Congress Toronto Canada 1996;II:733."},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820231309"},{"issue":"2","key":"e_1_2_7_32_2","first-page":"60","article-title":"Polyethylene oxide as a biomaterial.","volume":"6","author":"Merril EW","year":"1983","journal-title":"J Am Artif Intern Organs"},{"key":"e_1_2_7_33_2","first-page":"147","article-title":"Bioprosthetic heart valve calcification: Clinical features, pathobiology, and prospects for prevention.","volume":"2","author":"Levy RJ","year":"1986","journal-title":"CRC Crit Rev Biocompat"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1525-1594.2000.06348.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1525-1594.2000.06348.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T08:08:23Z","timestamp":1698566903000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1525-1594.2000.06348.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1046\/j.1525-1594.2000.06348.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1525-1594.2000.06348.x","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"type":"print","value":"0160-564X"},{"type":"electronic","value":"1525-1594"}],"subject":[],"published":{"date-parts":[[2000,2]]}}}