{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:32:51Z","timestamp":1775064771134,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,7,3]],"date-time":"2002-07-03T00:00:00Z","timestamp":1025654400000},"content-version":"vor","delay-in-days":32,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:p> <jats:bold>BACKGROUND<\/jats:bold> : Differential agglutination procedures and flow cytometric analysis have been used for detecting and quantitating mixed cell populations. For more than 20 years in our laboratory, a differential agglutination method using the coil planet centrifuge and polyclonal anti\u2010A or anti\u2010B has been used. However, it is now difficult to obtain polyclonal antisera, and it is unknown whether MoAbs can take the place of polyclonal antisera in the coil planet centrifuge method.<\/jats:p><jats:p> <jats:bold>STUDY DESIGN AND METHODS<\/jats:bold> : Polyclonal antisera and MoAbs were filled into a coil first and then unsensitized packed RBCs from ABO variants, and from chimeras and patients after ABO\u2010incompatible HPC trans\u2010 plantation (HPCT) were loaded. After centrifugation, agglutinated and nonagglutinated RBCs were collected, hemolyzed, and subjected to colorimetric analysis for quantitation.<\/jats:p><jats:p> <jats:bold>RESULTS<\/jats:bold> : ABO chimerism was quantitatively estimated with detection as low as 0.1 percent. ABO variants showed different patterns of agglutination and nonagglutination. The reconstitution status of the erythroid lineage after ABO\u2010mismatched HPCT was also quantitatively evaluated.<\/jats:p><jats:p> <jats:bold>CONCLUSION<\/jats:bold> : A modified coil planet centrifuge method is established by which ABO chimerism could quantitatively be analyzed and ABO variants identified to the same degree of accuracy as the other differential methods and flow cytometry. The monitoring of ABO chimerism might also help the diagnosis of early relapse or rejection after ABO incompatible HPCT.<\/jats:p>","DOI":"10.1046\/j.1537-2995.2002.00111.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T22:49:48Z","timestamp":1047509388000},"page":"702-710","source":"Crossref","is-referenced-by-count":6,"title":["Detection and quantitation of ABO RBC chimerism by a modified coil planet centrifuge method"],"prefix":"10.1111","volume":"42","author":[{"given":"Junko","family":"Takahashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiko","family":"Seno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Nakade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsunobu","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Sako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Yoshihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunio","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Tomiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiya","family":"Hirayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotoshi","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Tani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,7,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.29.3.267"},{"key":"e_1_2_6_3_2","unstructured":"Beattie KM. A seminar on problems encountered in pretransfusion tests. Washington DC: AABB 1972:160\u20101."},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.1968.tb02397.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1985.tb00793.x"},{"key":"e_1_2_6_6_2","first-page":"30S","article-title":"Using computer image processing to quantitate agglutinated red blood cells in mixed cell populations (abstract).","volume":"41","author":"Huang ST","year":"2001","journal-title":"Transfusion"},{"key":"e_1_2_6_7_2","first-page":"702","article-title":"The application of flow cytometry in analysis of erythrocyte antigen determination.","volume":"31","author":"Amemiya Y","year":"1990","journal-title":"Jpn J Clin Hematol"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb08410.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.d01-2138.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.1999.00201.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/003130299104954"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/212985a0"},{"key":"e_1_2_6_13_2","first-page":"455","article-title":"[Application of coil planet centrifuge in immunohematology].","volume":"71","author":"Kiyokawa H","year":"1980","journal-title":"Fukuoka Acta Med"},{"key":"e_1_2_6_14_2","unstructured":"Shibata H Takahashi J Seno T et al. Application of coil planet centrifuge for measurement of the ratio of A\u2010 and\/or B\u2010cells. 10th Regional Congress of the International Society of Blood Transfusion Western Pacific Region; 1999 Nov 11\u201014; Taipei Taiwan; p. 256."},{"issue":"1","key":"e_1_2_6_15_2","first-page":"1436","article-title":"The fetal i antigen is a good marker for the reconstitution of red blood cells after cord blood transplantation.","volume":"74","author":"Tani Y","year":"1998","journal-title":"Vox Sang"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1537-2995.2002.00111.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1537-2995.2002.00111.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T19:09:48Z","timestamp":1697224188000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1537-2995.2002.00111.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1046\/j.1537-2995.2002.00111.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1537-2995.2002.00111.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}