{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:56Z","timestamp":1772118776012,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2002,9,16]],"date-time":"2002-09-16T00:00:00Z","timestamp":1032134400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p> <jats:bold>BACKGROUND<\/jats:bold> : An anti\u2010Vel, nearly missed in antibody identification studies, and the effect of a commercially available rabbit RBC stroma (RESt, Immucor) adsorptions on eight anti\u2010Vel sera are reported. Anti\u2010Vel is an antibody to an antigen of high prevalence.<\/jats:p><jats:p> <jats:bold>CASE REPORT:<\/jats:bold> A 48\u2010year\u2010old woman with chronic vaginal bleeding presented with a Hct of 14.7 percent. The transfusion service was not informed of her history of anti\u2010Vel when she was transferred from another institution. Studies performed on an emergency request for transfusion were interpreted as a cold autoantibody as adsorption with a commercial source of RESt eliminated the reactivity. Stored anti\u2010Vel sera were tested by titration studies before and after adsorption with commercial RESt.<\/jats:p><jats:p> <jats:bold>RESULTS<\/jats:bold> : Serum from the index case did not react after adsorption with RESt at the transfusion service. Studies with the stored anti\u2010Vel indicated antibody adsorption with four of four samples at immediate spin (IS) and room temperature (RT) phases, four of eight samples at 37\u00b0C in albumin (ALB) phase, and four of eight samples at ALB\u2010IgG\u2010AGT phase. Variations in antibody reactivity were observed in the samples tested, but RESt adsorption diminished antibody reactivity in most samples. All eight stored sera demonstrated some reactivity in at least one phase after adsorption with RESt.<\/jats:p><jats:p> <jats:bold>CONCLUSION<\/jats:bold> : Anti\u2010Vel was completely or partially adsorbed by RESt. Caution should be used when interpreting cold agglutinins with this method. The manufacturer warns that uncommon alloantibodies may be adsorbed.<\/jats:p>","DOI":"10.1046\/j.1537-2995.2002.00187.x","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T15:18:58Z","timestamp":1034176738000},"page":"1180-1183","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010Vel reactivity diminished by adsorption with rabbit RBC stroma"],"prefix":"10.1111","volume":"42","author":[{"given":"Steven\u2003A.","family":"Mechanic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan\u2003L.","family":"Maurer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark\u2003J.","family":"Igoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna\u2003M.","family":"Kavitsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra\u2003T.","family":"Nance","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,9,16]]},"reference":[{"key":"e_1_2_7_2_2","unstructured":"RESt manufacturer's insert. Version 6\/99. Norcross (GA): Immucor 1999."},{"key":"e_1_2_7_3_2","first-page":"368","article-title":"[A novel blood factor Vel.]","volume":"7","author":"Sussman LN","year":"1952","journal-title":"Rev Hematol"},{"key":"e_1_2_7_4_2","first-page":"129","article-title":"The Vel blood group system.","volume":"6","author":"Sussman LN","year":"1972","journal-title":"Haematologia"},{"key":"e_1_2_7_5_2","unstructured":"Reid M Lomas\u2010Francis C. The blood group antigen facts book. New York: Academic Press 1997."},{"key":"e_1_2_7_6_2","unstructured":"Vengelen\u2010Tyler V (ed.). Technical manual of the American Association of Blood Banks 13th ed. Bethesda: AABB 1999."},{"key":"e_1_2_7_7_2","unstructured":"American National Red Cross. Immunohematology methods and procedures 1st edn. Washington (DC): American National Red Cross 1993."},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00521a034"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a134571"},{"key":"e_1_2_7_10_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.21307\/immunohematology-2019-927","article-title":"Misidentification of anti\u2010Vel due to inappropriate use of prewarming and adsorption techniques.","volume":"10","author":"Storry J","year":"1994","journal-title":"Immunohematology"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1998.7510070.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1983.23483276869.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1537-2995.2002.00187.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1537-2995.2002.00187.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1046\/j.1537-2995.2002.00187.x","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1537-2995.2002.00187.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T08:30:31Z","timestamp":1694421031000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1537-2995.2002.00187.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":11,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1046\/j.1537-2995.2002.00187.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1537-2995.2002.00187.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]}}}