{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T21:08:38Z","timestamp":1792357718162,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2003,8,15]],"date-time":"2003-08-15T00:00:00Z","timestamp":1060905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> Prolonged periods of marrow hypoplasia have been a problem in cord blood transplantation. DMSO is thought to produce osmotic shock to the progenitors when the thawed cells are infused into the patients. To solve this problem, a 2\u00d7 dilution method originally developed in the New York Blood Center showed earlier myeloid engraftment,<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#b1\"><jats:sup>1<\/jats:sup><\/jats:ext-link> although follow\u2010up clinical studies have not performed.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> To clarify the influence of the removal of DMSO by this method on the speed of engraftment in unrelated cord blood transplantation, 46 adult patients with cord blood units processed by the Tokyo Cord Blood Bank from September 1998 to March 31, 2002 were studied. Twenty\u2010four patients received 2.6 \u00b1 0.71 \u00d7 10<jats:sup>7<\/jats:sup> nucleated cells per kg without washing (nonwashed group), while 22 patients were received 2.7 \u00b1 0.52 \u00d7 10<jats:sup>7<\/jats:sup> nucleated cells per kg after 2\u00d7 dilution washing (washed group).<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> The cumulative incidence of engraftment was not significantly different between the two groups. Median neutrophil recovery (\u22655 \u00d7 10<jats:sup>9<\/jats:sup>\/L) in the nonwashed and washed groups was 26 and 25 days, respectively, and the median platelet recovery (\u226520 \u00d7 10<jats:sup>9<\/jats:sup>\/L) in patients with myeloid engraftment was 44 and 40 days, respectively (NS). On the other hand, the doses of CFCs and CD34+ cells showed the influence on myeloid and platelet recovery.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> A 2\u00d7 dilution after thawing cord blood did not result in the improvement of myeloid engraftment speed.<\/jats:p>","DOI":"10.1046\/j.1537-2995.2003.00486.x","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T21:15:46Z","timestamp":1104354946000},"page":"1285-1295","source":"Crossref","is-referenced-by-count":42,"title":["Wash\u2010out of DMSO does not improve the speed of engraftment of cord blood transplantation: follow\u2010up of 46 adult patients with units shipped from a single cord blood bank"],"prefix":"10.1111","volume":"43","author":[{"given":"Tokiko","family":"Nagamura\u2010Inoue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Shioya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiko","family":"Sugo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satomi","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhou","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Takada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Kodo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetaka","family":"Asano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuneo A.","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,8,15]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199607183350303"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3828"},{"key":"e_1_2_7_4_2","first-page":"123","article-title":"Recent trends in allogeneic bone marrow transplantation","author":"Gluckman E","year":"1989","journal-title":"Clin Transpl"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.7.1679.1679"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199811263392201"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200106143442410"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.10.2962"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-0734.2001.00034.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"crossref","first-page":"4665","DOI":"10.1182\/blood.V90.12.4665","article-title":"Placental and\/or umbilical cord blood. 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