{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:57:34Z","timestamp":1791219454263,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2003,8,13]],"date-time":"2003-08-13T00:00:00Z","timestamp":1060732800000},"content-version":"vor","delay-in-days":650,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiovasc electrophysiol"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p> <jats:bold>Electrophysiologic Studies in eNOS\u2010Deficient Mice.<\/jats:bold>\n          <jats:italic>Introduction:<\/jats:italic> Endothelial nitric oxide synthase (eNOS) mediates attenuation of the L\u2010type calcium channel and modulates myocyte contractility. Arrhythmogenic afterdepolarizations are seen in vitro in ouabain\u2010treated isolated myocytes from eNOSdeficient mice. The aim of these studies was to characterize the baseline electrophysiologic (EP) phenotype of eNOS\u2010deficient mice and their potential susceptibility to cardiac conduction abnormalities and inducible arrhythmias.<\/jats:p><jats:p> <jats:italic>Methods and Results:<\/jats:italic> Surface ECG and in vivo intracardiac EP studies were performed in 27 mice lacking the eNOS gene and 21 wild\u2010type littermate control mice. Baseline studies were performed in 10 eNOS\u2010deficient mice and 10 wild\u2010type controls. Subsequently, 17 eNOS\u2010deficient mice and 11 wild\u2010type controls were pretreated with digoxin, and ECG and EP testing were repeated. Data analysis revealed no significant differences in ECG intervals or cardiac conduction parameters, except sinus cycle length was higher in eNOS\u2010deficient mice than wild\u2010type mice (<jats:styled-content>P &lt; 0.01<\/jats:styled-content>). After digoxin pretreatment, 7 of 17 eNOS\u2010deficient mice had inducible ventricular tachycardia and 2 others had frequent ventricular premature beats, compared with only 3 of 11 wild\u2010type mice with inducible ventricular tachycardia. In addition, 2 digoxin\u2010treated eNOS\u2010deficient mice and 1 wild\u2010type mouse had inducible nonsustained atrial fibrillation.<\/jats:p><jats:p> <jats:italic>Conclusion:<\/jats:italic> Mice with a homozygous targeted disruption of the eNOS gene have slower heart rates but no other distinguishable EP characteristics under basal sedated conditions. Partial inhibition of the <jats:styled-content>Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup><\/jats:styled-content> ATPase pump with digoxin administration increases ventricular ectopic activity in eNOS<jats:sup>\u2013\/\u2013<\/jats:sup> mice, a phenotype analogous to afterdepolarizations seen in vitro in this eNOS\u2010deficient mouse model.<\/jats:p>","DOI":"10.1046\/j.1540-8167.2001.01295.x","type":"journal-article","created":{"date-parts":[[2003,8,13]],"date-time":"2003-08-13T11:22:11Z","timestamp":1060773731000},"page":"1295-1301","source":"Crossref","is-referenced-by-count":29,"title":["In Vivo Electrophysiologic Studies in Endothelial Nitric Oxide Synthase (eNOS)\u2010Deficient Mice"],"prefix":"10.1111","volume":"12","author":[{"given":"AMIT","family":"RAKHIT","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"COLIN T.","family":"MAGUIRE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HIROKO","family":"WAKIMOTO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOSEF","family":"GEHRMANN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GORDON K.","family":"LI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RALPH A.","family":"KELLY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"THOMAS","family":"MICHEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CHARLES I.","family":"BERUL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,8,13]]},"container-title":["Journal of Cardiovascular Electrophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1540-8167.2001.01295.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1540-8167.2001.01295.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T14:05:32Z","timestamp":1700402732000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1540-8167.2001.01295.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["10.1046\/j.1540-8167.2001.01295.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1540-8167.2001.01295.x","archive":["Portico"],"relation":{},"ISSN":["1045-3873","1540-8167"],"issn-type":[{"value":"1045-3873","type":"print"},{"value":"1540-8167","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11]]}}}