{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:09:22Z","timestamp":1762099762346},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":1303,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:p>We have investigated the kinetics of the amplification of the progenitor cell compartments (CFC) in haemopoietic organs during murine ontogenesis and compared the growth requirements of fetal and adult CFC. Two haemopoietic phases were recognized in the fetal liver (FL): an exponential growth phase, from 11.5 to 15.5\u2003d post conception (p.c.), during which the mean number of nucleated cells and of CFC in the FL increased from 4.9\u2003\u00d7\u200310<jats:sup>5<\/jats:sup> to 7.0\u2003\u00d7\u200310<jats:sup>7<\/jats:sup> and from 4.5\u2003\u00d7\u200310<jats:sup>3<\/jats:sup> to 2.7\u2003\u00d7\u200310<jats:sup>5<\/jats:sup>, respectively, and a recessive phase after 15.5\u2003d p.c., during which the CFC number in the FL gradually decreased, although some CFC were still detectable in the liver after birth. In serum\u2010deprived cultures, FL and adult marrow (AM) CFC had similar responses to GM\u2010CSF, and did not respond to G\u2010CSF or IL\u20103. In contrast, FL, but not AM, erythroid colonies grew Epo\u2010independently whereas SCF alone induced formation of maximal numbers of erythroid bursts from FL, but not from AM cells. The proliferative and differentiative effect of SCF alone on fetal cells was confirmed in serum\u2010deprived cultures of purified early progenitor cells isolated by cell sorting on the basis of multiple parameters from FL and AM light\u2010density cells. In culture of purified FL cells, SCF alone induced a similar amplification of total cells (maximal amplification at day 12: 800\u2013300\u2010fold) and total CFC (11\u201338\u2010fold of maximal amplification at day 6) to the combination of SCF plus IL\u20103 (1300\u2013800\u2010fold amplification of total cells and 31\u201388\u2010fold amplification of CFC). In contrast, SCF alone allowed only survival of purified AM early progenitor cells. Therefore FL early progenitor cells have an intrinsic higher potential than their adult counterpart to respond to SCF, confirming the potent role of this growth factor in the development of the murine haemopoietic system.<\/jats:p>","DOI":"10.1046\/j.1365-2141.1998.00775.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T06:02:20Z","timestamp":1047362540000},"page":"676-687","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Stem cell factor induces proliferation and differentiation of fetal progenitor cells in the mouse"],"prefix":"10.1111","volume":"101","author":[{"given":"Hitoshi","family":"Kurata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Carlo","family":"Mancini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Alespeiti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Rita","family":"Migliaccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Migliaccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"561","article-title":"A multiple staining procedure for the flow cytometric screening of monoclonal antibodies directed against murine pluripotent stem cells (CFU\u2010S)","volume":"14","author":"De Vries P.","year":"1988","journal-title":"Blood Cells"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091540408"},{"key":"e_1_2_6_4_2","first-page":"285","article-title":"Isolation and characterization of primitive hematopoietic progenitors of murine fetal liver","volume":"24","author":"Fujimoto K.","year":"1996","journal-title":"Experimental Hematology"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.10110"},{"key":"e_1_2_6_6_2","first-page":"19","article-title":"In vitro differentiation of B cells and myeloid cells from the early mouse embryo and its extraembryonic yolk sac","volume":"22","author":"Huang H.","year":"1994","journal-title":"Experimental Hematology"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90476-0"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/313806a0"},{"key":"e_1_2_6_9_2","first-page":"200","article-title":"Erythroid progenitor cells and stimulating factors during murine embryonic and fetal development","volume":"13","author":"Johnson G.R.","year":"1985","journal-title":"Experimental Hematology"},{"key":"e_1_2_6_10_2","first-page":"1011","article-title":"Long\u2010term repopulating abilities of enriched fetal liver stem cells measured by competitive repopulation","volume":"23","author":"Jordan C.T.","year":"1995","journal-title":"Experimental Hematology"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90061-I"},{"key":"e_1_2_6_12_2","unstructured":"Kaufman M.H.(1992)The Atlas of Mouse Development. 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