{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T15:19:31Z","timestamp":1759159171135},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,10,31]],"date-time":"2003-10-31T00:00:00Z","timestamp":1067558400000},"content-version":"vor","delay-in-days":2251,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p> <jats:italic>slyD<\/jats:italic> encodes a 196 amino acid polypeptide that is a member of the FKBP family of <jats:italic>cis\u2013trans<\/jats:italic> peptidyl\u2013prolyl isomerases (PPIases). <jats:italic>slyD<\/jats:italic> mutations affect plaque formation by the phage \u03c6X174 by blocking the action of the phage lysis protein E. Here we describe the selection of a set of spontaneous <jats:italic>slyD<\/jats:italic> mutations conferring resistance to the expression of gene <jats:italic>E<\/jats:italic> from a plasmid. These mutations occur disproportionately in residues of SlyD that, based on the structure of the prototype mammalian FKBP12, make ligand contacts with immunosuppressing drug molecules or are conserved in other FKBP proteins. A wide variation in the plating efficiency of \u03c6X174 on these <jats:italic>E<\/jats:italic>\u2003<jats:sup>R<\/jats:sup> strains is observed, relative to the parental, indicating that these alleles differ widely in residual SlyD activity. Moreover, it is found that <jats:italic>slyD<\/jats:italic> mutations cause significant growth rate defects in <jats:italic>Escherichia coli<\/jats:italic> B and C backgrounds. Finally, overexpression of <jats:italic>slyD<\/jats:italic> causes filamentation of the host. Thus, among the FKBP genes found in organisms across the evolutionary spectrum, <jats:italic>slyD<\/jats:italic> is unique in having three distinct drug\u2010independent phenotypes.<\/jats:p>","DOI":"10.1046\/j.1365-2958.1997.5201884.x","type":"journal-article","created":{"date-parts":[[2003,11,2]],"date-time":"2003-11-02T17:46:51Z","timestamp":1067795211000},"page":"1031-1046","source":"Crossref","is-referenced-by-count":52,"title":["Mutational analysis of <i>slyD<\/i>, an <i>Escherichia coli<\/i> gene encoding a protein of the FKBP immunophilin family"],"prefix":"10.1111","volume":"25","author":[{"given":"W. D.","family":"Roof","sequence":"first","affiliation":[]},{"given":"H. Q.","family":"Fang","sequence":"additional","affiliation":[]},{"given":"K. D.","family":"Young","sequence":"additional","affiliation":[]},{"given":"Jianling","family":"Sun","sequence":"additional","affiliation":[]},{"given":"Ry","family":"Young","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,10,31]]},"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2958.1997.5201884.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2958.1997.5201884.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1046\/j.1365-2958.1997.5201884.x","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2958.1997.5201884.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T12:02:13Z","timestamp":1694433733000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-2958.1997.5201884.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1046\/j.1365-2958.1997.5201884.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-2958.1997.5201884.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}