{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T20:25:03Z","timestamp":1768249503186,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"vor","delay-in-days":1489,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1998,2]]},"abstract":"<jats:p>The <jats:italic>Escherichia coli<\/jats:italic> sensory kinase, ArcB, possesses a histidine\u2010containing phosphotransfer (HPt) domain, which is implicated in the His\u2010Asp multistep phosphorelay. We searched for a presumed phosphohistidine phosphatase, if present, which affects the function of the HPt domain through its dephosphorylation activity. Using <jats:italic>in vivo<\/jats:italic> screening, we first identified a gene that appeared to interfere with the His\u2010Asp phosphorelay between the HPt domain and the receiver domain of OmpR, provided that the gene product was expressed through a multicopy plasmid. The cloned gene (named <jats:italic>sixA<\/jats:italic>) was found to encode a protein consisting of 161 amino acids, which has a noticeable sequence motif, an arginine\u2013histidine\u2013glycine (RHG) signature, at its N\u2010terminus. Such an RHG signature, which presumably functions as a nucleophilic phosphoacceptor, was previously found in a set of divergent enzymes, including eukaryotic fructose\u20102,6\u2010bisphosphatase, <jats:italic>E. coli<\/jats:italic> periplasmic phosphatase and <jats:italic>E. coli<\/jats:italic> glucose\u20101\u2010phosphate phosphatase, and ubiquitous phosphoglycerate mutase. Otherwise, the entire amino acid sequences of none of these enzymes resembles that of SixA. It was demonstrated <jats:italic>in vitro<\/jats:italic> that the purified SixA protein exhibited the ability to release the phosphoryl group from the HPt domain of ArcB, but the mutant protein lacking the crucial histidine residue in the RHG signature did not. Evidence was also provided that a deletion mutation of <jats:italic>sixA<\/jats:italic> on the chromosome affected the <jats:italic>in vivo<\/jats:italic> phosphotransfer signalling. These results support the view that SixA is capable of functioning as a phosphohistidine phosphatase that may be implicated in the His\u2010Asp phosphorelay through regulating the phosphorylation state of the HPt domain.<\/jats:p>","DOI":"10.1046\/j.1365-2958.1998.00703.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:16:11Z","timestamp":1047467771000},"page":"573-585","source":"Crossref","is-referenced-by-count":62,"title":["An <i>Escherichia coli<\/i> protein that exhibits phosphohistidine phosphatase activity towards the HPt domain of the ArcB sensor involved in the multistep His\u2010Asp phosphorelay"],"prefix":"10.1111","volume":"27","author":[{"given":"Tomoaki","family":"Ogino","sequence":"first","affiliation":[]},{"given":"Masahiro","family":"Matsubara","sequence":"additional","affiliation":[]},{"given":"Naoki","family":"Kato","sequence":"additional","affiliation":[]},{"given":"Yoshihiro","family":"Nakamura","sequence":"additional","affiliation":[]},{"given":"Takeshi","family":"Mizuno","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"8563","DOI":"10.1016\/S0021-9258(18)81828-2","article-title":"Transfer of a phosphoryl group between two regulatory proteins involved in osmoregulatory expression of the ompF ad ompC genes in Escherichia coli","volume":"264","author":"Aiba H.","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71647-5"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.8.3416"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80158-0"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.17.6671"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.60.070191.002153"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06849.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/0092-8674(91)90238-T","article-title":"Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay","volume":"65","author":"Burbulys D.","year":"1991","journal-title":"Cell"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8211181"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.17.5429-5435.1997"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90218-7"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90489-8"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","unstructured":"Hoch J.A.&Silhavy T.J.(1995)Two\u2010Component Signal Transduction Washington DC: American Society for Microbiology.","DOI":"10.1128\/9781555818319"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7569898"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81076-4"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06850.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)80480-3"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00642.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5289.982"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81914-5"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_23_2","unstructured":"Lynch A.S.&Lin E.C.C.(1996) Responses in molecular oxygen. 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