{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T03:05:49Z","timestamp":1772766349165,"version":"3.50.1"},"reference-count":43,"publisher":"Institution of Engineering and Technology (IET)","issue":"3","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>\n                    Magnetic nanoparticles (MNPs) were prepared by co\u2010precipitation of Fe\n                    <jats:sup>+3<\/jats:sup>\n                    \/Fe\n                    <jats:sup>+2<\/jats:sup>\n                    (2:1; molar ratio) with a mean size of 40\u2009\u00b1\u20095\u00a0nm. Scanning electron microscopy, dynamic light scattering, X\u2010ray diffraction, vibrating sample magnetometer and thermogravimetric analysis were used to characterise the MNPs. The MNPs and also hydroxylated nonporous glass beads were similarly functionalised with the aid of glutaraldehyde to be cross\u2010linked to lipase originated from\n                    <jats:italic>Thermomyces lanuginosus<\/jats:italic>\n                    (TLL). The yield and efficiency for immobilised lipase on the MNPs were calculated as 72\u2009\u00b1\u20092.4 and 63\u2009\u00b1\u20093.5%, whereas a yield and efficiency of 60\u2009\u00b1\u20092.1 and 55\u2009\u00b1\u20094.1%, respectively, were measured for the corresponding parameters of the immobilised enzyme on the glass beads. When the immobilised TLL was compared with its free form, Michaelis\u2013Menten kinetics indicated an insignificant change in the Michaelis constant (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    ) and a drastic decrease in the maximum substrate conversion rate (\n                    <jats:italic>V<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    ). The immobilised TLL and six other commercial lipases were then checked for regioselective acetylation of prednisolone. The highest and lowest yields of the product were observed for Novozym 435 and immobilised TLL on the glass beads, respectively. Immobilised TLL retained its bioacetylation activity of prednisolone in five successive catalytic processes.\n                  <\/jats:p>","DOI":"10.1049\/iet-nbt.2012.0025","type":"journal-article","created":{"date-parts":[[2013,9,23]],"date-time":"2013-09-23T10:24:43Z","timestamp":1379931883000},"page":"100-108","source":"Crossref","is-referenced-by-count":17,"title":["Immobilisation of lipase on the surface of magnetic nanoparticles and non\u2010porous glass beads for regioselective acetylation of prednisolone"],"prefix":"10.1049","volume":"7","author":[{"given":"Sabrieh","family":"Ghasemi","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Science Shahid Beheshti University, G.C. Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armin","family":"Sadighi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biotechnology Faculty of Pharmacy and Biotechnology Research Center Tehran University of Medical Sciences P.O. Box 14155\u20136451 Tehran 14174 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjan","family":"Heidary","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Shahid Beheshti University, G.C. Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Bozorgi\u2010Koushalshahi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biotechnology Faculty of Pharmacy and Biotechnology Research Center Tehran University of Medical Sciences P.O. Box 14155\u20136451 Tehran 14174 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohreh","family":"Habibi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Shahid Beheshti University, G.C. Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Ali","family":"Faramarzi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biotechnology Faculty of Pharmacy and Biotechnology Research Center Tehran University of Medical Sciences P.O. 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