{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:33:54Z","timestamp":1761676434271,"version":"build-2065373602"},"reference-count":38,"publisher":"Institution of Engineering and Technology (IET)","issue":"3","license":[{"start":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T00:00:00Z","timestamp":1469664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>The present study focus on optical sensing of breast cancer antigen 15.3 (CA 15.3) using cadmium sulphide quantum dot (CdS\u2010QD) in saline and serum samples spiked with antigen. The surface of CdS\u2010QD was modified by cysteamine capping followed by tagging of CA 15.3 antibody. The samples were characterised using UV\u2010visible absorption spectroscopy (UV\u2010VIS Spectroscopy), Fourier transform infrared spectroscopy (FTIR), high\u2010resolution transmission electron microscopy (HRTEM) attached with energy\u2010dispersive X\u2010ray spectroscopy, phase contrast inverted epi\u2010fluorescence microscopy and photoluminescence (PL) spectrophotometry (EDS). The CdS\u2010QD showed a mean diameter of 3.02\u2009\u00b1\u20090.6 nm. The complex formed after antigen\u2010antibody interaction resulted in distinguishable optical and fluorescence intensity with respect to varying concentration of antigen. The PL study revealed that CA 15.3 antibody labelled CdS QD can detect CA 15.3 tumour marker even at very low concentration of 0.002\u00a0KU\/L with a constant response time of 15 min. This study clearly indicates that detection of CA 15.3 at low concentration is possible using surface modified CdS QD in serum samples and can find immense applications in biosensor development for detection of breast cancer marker similar to various automated detection kits available in market.<\/jats:p>","DOI":"10.1049\/iet-nbt.2016.0012","type":"journal-article","created":{"date-parts":[[2016,6,27]],"date-time":"2016-06-27T13:06:56Z","timestamp":1467032816000},"page":"268-276","source":"Crossref","is-referenced-by-count":16,"title":["Optical detection of CA 15.3 breast cancer antigen using CdS quantum dot"],"prefix":"10.1049","volume":"11","author":[{"given":"Venugopal","family":"Elakkiya","sequence":"first","affiliation":[{"name":"Nanobiotechnology Laboratory PSG Institute of Advanced Studies Peelamedu Coimbatore 641004 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mridula Prakash","family":"Menon","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory PSG Institute of Advanced Studies Peelamedu Coimbatore 641004 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devaraj","family":"Nataraj","sequence":"additional","affiliation":[{"name":"Department of Physics Bharathiar University Coimbatore 641046 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pullithadathil","family":"Biji","sequence":"additional","affiliation":[{"name":"Nanosensor Laboratory PSG Institute of Advanced Studies Peelamedu Coimbatore 641004 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendran","family":"Selvakumar","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Laboratory PSG Institute of Advanced Studies Peelamedu Coimbatore 641004 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"265","published-online":{"date-parts":[[2016,7,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2015.07.008"},{"key":"e_1_2_5_3_1","first-page":"1290","article-title":"Breast cancer screening policies in developing countries: a costeffectiveness analysis for India","volume":"100","author":"Quirine L.O.","year":"2013","journal-title":"J. Natl. Cancer Inst."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1414-7"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.17322"},{"key":"e_1_2_5_7_1","first-page":"12","article-title":"Molecular markers for breast cancer: prediction on tumor behavior","volume":"2014","author":"Hirata B.K.","year":"2014","journal-title":"Dis. Markers"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.059832"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-220"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0230822"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/43.4.585"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0133830"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/44.4.765"},{"issue":"125","key":"e_1_2_5_14_1","first-page":"752","article-title":"Performance characteristics of seven automated CA 15.3 assays","author":"Patricia R.S.","year":"2006","journal-title":"Am. J. Clin. Pathol."},{"issue":"11","key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"811","DOI":"10.5185\/amlett.2013.3437","article-title":"Quantum dots for diagnosis of cancers review article","volume":"4","author":"Malik P.","year":"2013","journal-title":"Adv. Mat. Lett."},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.5b00321"},{"key":"e_1_2_5_17_1","first-page":"51","article-title":"Recent developments in cancer therapy by the use of nanotechnology","volume":"4","author":"Kumar B.","year":"2009","journal-title":"Dig. J. Nanomater Biostruct."},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-22942-3_7"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4MH00066H"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/676839"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/s100100428"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1381-5148(02)00197-9"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","unstructured":"BostickR.M.KongK.Y.AhearnT.U.et al.: \u2018Detecting and quantifying biomarkers of risk for colorectal cancer using quantum dots and novel image analysis algorithms\u2019.Engineering in Medicine and Biology Society EMBS'06. 28th Annual Int. Conf. of the IEEE 2006 pp.3313\u20133316","DOI":"10.1109\/IEMBS.2006.259745"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie201826e"},{"key":"e_1_2_5_25_1","doi-asserted-by":"crossref","unstructured":"XiaL.XuL.Songet al.:\u2018CdS quantum dots modified CuO inverse opal electrodes for ultrasensitive electrochemical and photoelectrochemical biosensor\u2019 Scientific Reports 2015 5 p.12Article number: 10838","DOI":"10.1038\/srep10838"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2012.10.052"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1049\/iet-nbt.2010.0001"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-010-0323-3"},{"issue":"11","key":"e_1_2_5_29_1","first-page":"2245","article-title":"J. phys. chem. solids","volume":"64","author":"Ochoa\u2010Landn R.","year":"2003","journal-title":"J. Phys. Chem. Solids"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.25.1409"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.10.099"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-013-1047-y"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2003.08.017"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AY02472B"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.14573\/altex.1309181"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.10.030"},{"issue":"11","key":"e_1_2_5_37_1","first-page":"1703","article-title":"Design of aptamer\u2010gold nanoparticles based colorimetric assay for the early diagnosis of breast tumor","volume":"3","author":"Agnihotri N.P.","year":"2014","journal-title":"IJSR"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2006.085126"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201002349"}],"container-title":["IET Nanobiotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1049\/iet-nbt.2016.0012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1049\/iet-nbt.2016.0012","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ietresearch.onlinelibrary.wiley.com\/doi\/pdf\/10.1049\/iet-nbt.2016.0012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:09:53Z","timestamp":1761674993000},"score":1,"resource":{"primary":{"URL":"https:\/\/ietresearch.onlinelibrary.wiley.com\/doi\/10.1049\/iet-nbt.2016.0012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,28]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1049\/iet-nbt.2016.0012"],"URL":"https:\/\/doi.org\/10.1049\/iet-nbt.2016.0012","archive":["Portico"],"relation":{},"ISSN":["1751-8741"],"issn-type":[{"type":"print","value":"1751-8741"}],"subject":[],"published":{"date-parts":[[2016,7,28]]}}}