{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T15:30:42Z","timestamp":1766158242368,"version":"build-2065373602"},"reference-count":54,"publisher":"Institution of Engineering and Technology (IET)","issue":"3","license":[{"start":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T00:00:00Z","timestamp":1545264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p>\n                    A facile and green synthesis of platinum nanoparticles [gum kondagogu platinum nanoparticles (GKPtNP)] using biopolymer\u2010 gum kondagogu was developed. The formation of GKPtNP was confirmed by ultraviolet (UV)\u2013visible spectroscopy, scanning electron microscopy\u2013energy dispersive X\u2010ray spectroscopy, transmission electron microscopy, X\u2010ray diffraction, Zeta potential, Fourier transform infrared, inductively coupled plasma mass spectroscopy. The formed GKPtNP are well dispersed, homogeneous with a size of 2\u20134\u2009\u00b1\u20090.50\u00a0nm, having a negative zeta potential (\u221246.1\u00a0mV) indicating good stability. 5\u2010Fluorouracil (5FU) was loaded onto the synthesised GKPtNP, which leads to the development of a new combination of nanomedicine (5FU\u2013GKPtNP). The in vitro drug release studies of 5FU\u2013GKPtNP in pH 7.4 showed a sustained release profile over a period of 120\u00a0min.\n                    <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n                    induced in vitro potato tumour bioassay was employed for screening the anti\u2010tumour potentials of GKPtNP, 5FU, and 5FU\u2013GKPtNP. The experimental results suggested a complete tumour inhibition by 5FU\u2013GKPtNP at a lower concentration than the GKPtNP and 5FU. Furthermore, the mechanism of anti\u2010tumour activity was assessed by their interactions with DNA using agarose gel electrophoresis and UV\u2010spectroscopic analysis. The electrophoresis results revealed that the 5FU\u2013GKPtNP totally diminishes DNA and the UV\u2010spectroscopic analysis showed a hyperchromic effect with red shift indicating intercalation type of binding with DNA. Over all, the present study revealed that the combined exposure of the nanoformulation resulted in the enhanced anti\u2010tumour effect\n                    <jats:italic>.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1049\/iet-nbt.2018.5171","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T22:38:33Z","timestamp":1540939113000},"page":"282-292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Synthesis, characterisation and anti\u2010tumour activity of biopolymer based platinum nanoparticles and 5\u2010fluorouracil loaded platinum nanoparticles"],"prefix":"10.1049","volume":"13","author":[{"given":"Deepika","family":"Godugu","sequence":"first","affiliation":[{"name":"Department of Biochemistry University College of Science, Osmania University Hyderabad 500 007 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sashidhar Rao","family":"Beedu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University College of Science, Osmania University Hyderabad 500 007 Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"265","published-online":{"date-parts":[[2018,12,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr030698"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.clpt.6100400"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/la9814283"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3736\/jcim20120613"},{"key":"e_1_2_7_6_1","first-page":"42","article-title":"Platinum nanoparticles synthesis by sonoelectrochemical methods","volume":"47","author":"Obreja L.","year":"2010","journal-title":"Mater. 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