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Ultraviolet\u2013visible absorption spectra, photoluminescence (PL) spectra, and high\u2010resolution transmission electron microscopy analysis showed that the biosynthesised CdSe QDs have an average size of 3.1\u00a0nm, the excellent optical properties with fluorescence emission around 494\u00a0nm, and the good crystallinity. It was found that addition of 80\u00a0mg of mercaptosuccinic acid resulted in the formation of CdSe QDs with highest PL intensity. Furthermore, Fourier\u2010transform infrared spectra of as\u2010synthesised CdSe QDs confirmed the presence of a surface protein capping layer. 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