{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T13:16:47Z","timestamp":1780579007634,"version":"3.54.1"},"reference-count":52,"publisher":"Institution of Engineering and Technology (IET)","issue":"1","license":[{"start":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T00:00:00Z","timestamp":1576454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:p>\n                    <jats:italic>Ureaplasma urealyticum<\/jats:italic>\n                    (uu) is one of the most common agents of urogenital infections and is associated with complications such as infertility, spontaneous abortion and other sexually transmitted diseases. Here, a DNA sensor based on oligonucleotide target\u2010specific gold nanoparticles (AuNPs) was developed, in which the dispersed and aggregated states of oligonucleotide\u2010functionalised AuNPs were optimised for the colorimetric detection of a polymerase chain reaction (PCR) amplicon of\n                    <jats:italic>U. urealyticum<\/jats:italic>\n                    DNA. A non\u2010cross\u2010linking approach utilising a single Au\u2010nanoprobe specific of the urease gene was utilised and the effect of a PCR product concentration gradient evaluated. Results from both visual and spectral analyses showed that target\u2013Au\u2010nanoprobe hybrids were stable against aggregation after adding the inducer. Furthermore, when a non\u2010target PCR product was used, the peak position shifted and salt\u2010induced aggregation occurred. The assay's limit of detection of the assay was 10\u00a0ng with a dynamic range of 10\u201360\u00a0ng. This procedure provides a rapid, facile and low\u2010cost detection format, compared to methods currently used for the identification of\n                    <jats:italic>U. urealyticum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1049\/iet-nbt.2019.0088","type":"journal-article","created":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T22:43:30Z","timestamp":1570488210000},"page":"19-24","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Colorimetric\u2010based detection of\n                    <i>Ureaplasma urealyticum<\/i>\n                    using gold nanoparticles"],"prefix":"10.1049","volume":"14","author":[{"given":"Nahid","family":"Ghorbanzadeh","sequence":"first","affiliation":[{"name":"Student Research Committee Qazvin University of Medical Sciences Qazvin Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Peymani","sequence":"additional","affiliation":[{"name":"Medical Microbiology Research Center Qazvin University of Medical Sciences Qazvin Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Ahmadpour\u2010Yazdi","sequence":"additional","affiliation":[{"name":"Medical Biotechnology Department Faculty of Paramedical Sciences Qazvin University of Medical Sciences Qazvin Iran"},{"name":"Medical Microbiology Research Center Qazvin University of Medical Sciences Qazvin Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"265","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3345\/kjp.2010.53.12.989"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2005.04.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2014-0112"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa8419"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-001-0665-1"},{"issue":"4","key":"e_1_2_7_7_1","first-page":"80","article-title":"Clinical observation of sparfloxacin in the treatment of Ureaplasma Urealyticum prostatitis","volume":"36","author":"Huang R.","year":"2016","journal-title":"Chinese J. 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