{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T14:22:43Z","timestamp":1783088563494,"version":"3.54.6"},"reference-count":29,"publisher":"Institution of Engineering and Technology (IET)","issue":"6","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:p>Cisplatin treatment results in acute kidney injury (AKI) by the phosphorylation of mixed lineage kinase domain\u2010like protein (MLKL). The knockout of MLKL, which is a principle mediator of necroptosis, is believed to alleviate the AKI symptoms. The present study was aimed to improve the therapeutic efficacy in AKI. For this purpose, miR\u2010500a\u20103P was identified as appropriate miRNA therapeutics and loaded in liposome delivery carrier. The authors have showed that the miR\u2010LIP directly controls the expression of RIPK3 and MLKL \u2013 a modulator of necroptosis and thereby reduces the severity of kidney injury. The miR\u2010LIP significantly controlled the phosphorylation of MLKL compared to that of CDDP\u2010treated HK2 cells. Similar results are observed with RIPK3. The miR\u2010LIP has also been demonstrated to control the inflammatory response in tubular cells. Western blot analysis further revealed that the phosphorylation of P\u201065 was mainly responsible for the inflammatory response and miR\u2010LIP significantly decreased the CDDP\u2010induced NF\u2010kB phosphorylation. Overall, the present study explored the molecular mechanism behind the necroptosis in AKI and potential of miRNA in targeting MLKL pathways. Study further highlights the potential advantage of liposome as a delivery carrier for miRNA therapeutics.<\/jats:p>","DOI":"10.1049\/iet-nbt.2019.0247","type":"journal-article","created":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T22:34:57Z","timestamp":1584484497000},"page":"465-469","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Functional role of microRNA\u2010500a\u20103P\u2010loaded liposomes in the treatment of cisplatin\u2010induced AKI"],"prefix":"10.1049","volume":"14","author":[{"given":"Suhua","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Kidney Disease Suzhou Kowloon Hospital Affiliated to Medical College of Shanghai Jiaotong University Suzhou Jiangsu 215028 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaixin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Kidney Disease Suzhou Kowloon Hospital Affiliated to Medical College of Shanghai Jiaotong University Suzhou Jiangsu 215028 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixin","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Kidney Disease Suzhou Kowloon Hospital Affiliated to Medical College of Shanghai Jiaotong University Suzhou Jiangsu 215028 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology The First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"265","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2017.00078"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.02140215"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2016-0319"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/967826"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc13133"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181feeb15"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-052016-100247"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10274"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2883"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.37"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2015.10.008"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07228"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005825"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1997"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-2001-y"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.03.016"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-016-9393-8"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2014.05.009"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.070"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b01611"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8TB00489G"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2019.1642421"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.9b00982"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305538110"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2017.04.043"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2017.115"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.06.033"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000490807"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-017-0568-3"}],"container-title":["IET Nanobiotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1049\/iet-nbt.2019.0247","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1049\/iet-nbt.2019.0247","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ietresearch.onlinelibrary.wiley.com\/doi\/pdf\/10.1049\/iet-nbt.2019.0247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:54:36Z","timestamp":1761692076000},"score":1,"resource":{"primary":{"URL":"https:\/\/ietresearch.onlinelibrary.wiley.com\/doi\/10.1049\/iet-nbt.2019.0247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,13]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1049\/iet-nbt.2019.0247"],"URL":"https:\/\/doi.org\/10.1049\/iet-nbt.2019.0247","archive":["Portico"],"relation":{},"ISSN":["1751-8741"],"issn-type":[{"value":"1751-8741","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7,13]]},"assertion":[{"value":"2019-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}