{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T04:29:40Z","timestamp":1767155380258,"version":"build-2238731810"},"reference-count":52,"publisher":"Institution of Engineering and Technology (IET)","issue":"4","license":[{"start":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T00:00:00Z","timestamp":1584057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81502255"],"award-info":[{"award-number":["81502255"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21603084"],"award-info":[{"award-number":["21603084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2017BB015"],"award-info":[{"award-number":["ZR2017BB015"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2016BP10"],"award-info":[{"award-number":["ZR2016BP10"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019PB026"],"award-info":[{"award-number":["ZR2019PB026"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008853","name":"Jining Medical University","doi-asserted-by":"publisher","award":["JYP20418KJ17"],"award-info":[{"award-number":["JYP20418KJ17"]}],"id":[{"id":"10.13039\/501100008853","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p>\n                    The binding reaction of reduced graphene oxide\u2013silver nanocomposites (rGO\u2013AgNCs) with calf thymus single\u2010stranded DNA (ssDNA) was studied by ultraviolet\u2013visible absorption, fluorescence spectroscopy and circular dichroism (CD), using berberine hemisulphate (BR) dye as a fluorescence probe. The absorbance of ssDNA increases, but the fluorescence intensity is quenched with the addition of rGO\u2013AgNCs. The binding of rGO\u2013AgNCs with ssDNA was able to increase the quenching effects of BR and ssDNA, and induce the changes in CD spectra. All of the evidence indicated that there was a relatively strong interaction between ssDNA and rGO\u2013AgNCs. The data obtained from fluorescence experiments revealed that the quenching process of ssDNA caused by rGO\u2013AgNCs is primarily due to complex formation, i.e. static quenching. The increasing trend of the binding equilibrium constant (\n                    <jats:italic>K<\/jats:italic>\n                    a) with rising temperature indicated that the binding process was an endothermic reaction. The calculated thermodynamic parameters showed that the binding process was thermodynamically spontaneous, and hydrophobic association played predominant roles in the binding of ssDNA to the surface of rGO\u2013AgNCs.\n                  <\/jats:p>","DOI":"10.1049\/iet-nbt.2019.0377","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T21:16:34Z","timestamp":1582751794000},"page":"308-313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Studies on binding of single\u2010stranded DNA with reduced graphene oxide\u2013silver nanocomposites"],"prefix":"10.1049","volume":"14","author":[{"given":"Xi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou 510006 Guangdong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Program for Scientific Research Innovation Team in Precision Medicine of Gynecologic Oncology Affiliated Hospital of Jining Medical University Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1371-278X","authenticated-orcid":false,"given":"Yunfei","family":"Wang","sequence":"additional","affiliation":[{"name":"Program for Scientific Research Innovation Team in Precision Medicine of Gynecologic Oncology Affiliated Hospital of Jining Medical University Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongyu","family":"Du","sequence":"additional","affiliation":[{"name":"College of Basic Medical, Jining Medical University Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuyan","family":"Mao","sequence":"additional","affiliation":[{"name":"College of Basic Medical, Jining Medical University Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dezhi","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Liaocheng University Liaocheng 252059 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Basic Medical, Jining Medical University Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Public Health, Jining Medical College Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyu","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Basic Medical, Jining Medical University Jining 272067 Shandong Province People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"265","published-online":{"date-parts":[[2020,3,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA00733D"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA00344D"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA00010K"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6090259"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40242-019-8327-y"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.5b00052"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20058856"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/am500641d"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/CH18148"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.materresbull.2014.02.015"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b06052"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201801300"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10973-019-08153-4"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8NJ05800H"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.2693"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11051-019-4701-4"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200901479"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja103169v"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn300217z"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.05.043"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.7b00600"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn201560b"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/am505677x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2011.01.008"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/am4000485"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.7b00074"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf303698k"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcc.6b05396"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsphotonics.6b00062"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b11001"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja306854d"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NR08465B"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn402472k"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-013-1177-y"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2014.03.022"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2014.02.004"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2013.03.015"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2005.12.034"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2014.06.032"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/10601329508010296"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0308-8146(03)00276-0"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866043406814"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2707909"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2014.06.001"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2017.01.003"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tca.2009.03.017"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.5b00436"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2014.02.009"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2016.02.103"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf2019006"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00514a017"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2009.11.015"}],"updated-by":[{"DOI":"10.1049\/nbt2.12066","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T00:00:00Z","timestamp":1637884800000}}],"container-title":["IET Nanobiotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1049\/iet-nbt.2019.0377","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1049\/iet-nbt.2019.0377","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ietresearch.onlinelibrary.wiley.com\/doi\/pdf\/10.1049\/iet-nbt.2019.0377","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:50:48Z","timestamp":1761691848000},"score":1,"resource":{"primary":{"URL":"https:\/\/ietresearch.onlinelibrary.wiley.com\/doi\/10.1049\/iet-nbt.2019.0377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,13]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1049\/iet-nbt.2019.0377"],"URL":"https:\/\/doi.org\/10.1049\/iet-nbt.2019.0377","archive":["Portico"],"relation":{},"ISSN":["1751-8741"],"issn-type":[{"value":"1751-8741","type":"print"}],"subject":[],"published":{"date-parts":[[2020,3,13]]},"assertion":[{"value":"2020-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}