{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T06:04:19Z","timestamp":1778997859095,"version":"3.51.4"},"reference-count":68,"publisher":"Institution of Engineering and Technology (IET)","issue":"9","license":[{"start":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T00:00:00Z","timestamp":1604275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:p>\n                    Listeriosis through contaminated food is one of the leading causes of premature deaths in pregnant women and new born babies. Here, the authors have developed a magnetosomes\u2010based biosensor for the rapid, sensitive, specific and cost\u2010effective detection of\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    from food sample. Magnetosomes were extracted from\n                    <jats:italic>Magnetospirillum sp.<\/jats:italic>\n                    RJS1 and then directly bound to anti\u2010Listeriolysin antibody (0.25\u20131 \u00b5g\/ml), confirmed in spectroscopy. Listeriolysin (LLO) protein (0.01\u20137 \u00b5g\/ml) was optimised in enzyme\u2010linked immunosorbent assay. Magnetosomes was conjugated with LLO antibody (0.25 \u00b5g\/ml) in optimum concentration to detect LLO protein (0.01 \u00b5g\/ml). Magnetosomes\u2013LLO antibody complex was 25% cost effective. The magnetosomes\u2013LLO antibody complex was directly stabilised on screen printed electrode using external magnet. The significant increase in resistance (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sub>CT<\/jats:sub>\n                    value) on the electrode surface with increase in concentration of LLO protein was confirmed in impedance spectroscopy. The\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    contaminated milk and water sample were processed and extracted LLO protein was detected in the biosensor. The specificity of the biosensor was confirmed in cross\u2010reactivity assay with other food pathogens. The detection limit of 10\n                    <jats:sup>1<\/jats:sup>\n                    Cfu\/ml in both water and milk sample manifests the sensitive nature of the biosensor. The capture efficiency and field emission scanning electron microscopy confirmed positive interaction of Listeria cells with magnetosomes\u2013antibody complex.\n                  <\/jats:p>","DOI":"10.1049\/iet-nbt.2020.0091","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T21:20:04Z","timestamp":1604352004000},"page":"839-850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of magnetosomes\u2010based biosensor for the detection of\n                    <i>Listeria monocytogenes<\/i>\n                    from food sample"],"prefix":"10.1049","volume":"14","author":[{"given":"Sumana","family":"Sannigrahi","sequence":"first","affiliation":[{"name":"Marine Biotechnology and Bioproducts Lab School of Biosciences and Technology VIT\u2010Vellore 632014 Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5213-7314","authenticated-orcid":false,"given":"Shiva Kumar","family":"Arumugasamy","sequence":"additional","affiliation":[{"name":"Electrodics and Electrocatalysis Division CSIR \u2013 Central Electrochemical Research Institute Karaikudi 630003 Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayaraman","family":"Mathiyarasu","sequence":"additional","affiliation":[{"name":"Electrodics and Electrocatalysis Division CSIR \u2013 Central Electrochemical Research Institute Karaikudi 630003 Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishnamurthy","family":"Suthindhiran","sequence":"additional","affiliation":[{"name":"Marine Biotechnology and Bioproducts Lab School of Biosciences and Technology VIT\u2010Vellore 632014 Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"265","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"69","article-title":"Dairy pathogens: characteristics and impact","volume":"16","author":"Papademas P.","year":"2015","journal-title":"Dairy Microbiol., Pract. 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