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Almost, all cervical cancer cases are associated with high\u2010risk human papillomavirus (HR\u2010HPV) infection. Accurate viral diagnosis has so far relied on the extraction of adequate amounts of DNA from formalin\u2010fixed, paraffin\u2010embedded (FFPE) tissue samples. Till now, no specific and sensitive DNA purification method has been introduced for the extraction of HR\u2010HPV from FFPE tissue. Since the commercially available purification kits are not sensitive and specific enough for HR\u2010HPV DNA targets, in this study, a DNA purification method was designed based on AuNPs to purify sufficient amounts of HR\u2010HPV DNA from cervical cancer tissue samples. AuNPs were coated with a series of oligonucleotide probes to hybridize to specific DNA sequences of HR\u2010HPV genotypes. Results showed that 733 out of 800 copies of type\u2010specific HPV DNA were recovered with complete specificity, compared to 36 copies with a standard commercial kit (Qiagen FFPE). 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