{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:09:05Z","timestamp":1780484945462,"version":"3.54.1"},"reference-count":32,"publisher":"Institution of Engineering and Technology (IET)","issue":"5","license":[{"start":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T00:00:00Z","timestamp":1620777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The present study investigates the potential role of dioscin (DIO) in the lipopolysaccharide (LPS)\u2010induced kidney injury. For this purpose, DIO\u2010loaded zein nanoparticles (DIO\u2010ZNPs) were formulated and evaluated for physicochemical parameters. The DIO\u2010ZNPs exhibited a controlled release of drug compared with that of the free drug suspension. Results showed that the cell viability of NRK\u201052E consistently decreased with the increase in LPS from 0.01\u00a0\u00b5g\/ml to 2\u00a0\u00b5g\/ml. When compared with LPS, DIO\u2010induced NPs showed 1.10\u2010, 1.32\u2010, 1.57\u2010 and 1.92\u2010fold increase in the cell viability for concentrations of 20\u00a0\u00b5g\/ml, 50\u00a0\u00b5g\/ml, 100\u00a0\u00b5g\/ml and 200\u00a0\u00b5g\/ml, respectively. DIO\u2010ZNPs exhibited the most remarkable recovery in the cell proliferation compared with free DIO as shown by the cellular morphology analysis. Furthermore, Annexin\u2010V staining analysis showed that the LPS\u2010treated cells possess the lowest green fluorescence indicating fewer viable cells, whereas DIO\u2010ZNPs exhibited the maximum green fluorescence comparable with that of the non\u2010treated cells indicating maximum cell viability. Furthermore, the results show that DIO\u2010ZNPs significantly increased the expression of miR\u2010let\u20107i in the epithelial kidney cells, whereas the expression levels of TLR4 were significantly downregulated compared with that of the LPS\u2010treated cells. In conclusion, miR\u2010let\u20107i could be an interesting therapeutic target and nanoparticle\u2010based DIO could be a potential candidate in the management of acute kidney injury<\/jats:p>","DOI":"10.1049\/nbt2.12051","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T01:19:20Z","timestamp":1620868760000},"page":"465-472","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dioscin\u2010loaded zein nanoparticles alleviate lipopolysaccharide\u2010induced acute kidney injury via the microRNA\u2010let 7i signalling pathways"],"prefix":"10.1049","volume":"15","author":[{"given":"Yun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuangen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changda","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiequn","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhai","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Renal Medicine Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"265","published-online":{"date-parts":[[2021,5,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04360514"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004090740"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005091017"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005060668"},{"key":"e_1_2_7_6_1","first-page":"1","article-title":"Acute kidney injury in sepsis","volume":"3","author":"Bellomo R.","year":"2017","journal-title":"Intensive Care Med"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra032401"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2008.02007.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc6823"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.9b00982"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702633200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2011.08.005"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702553104"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1391"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.08.011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2009.09.046"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1250461"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09713"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-013-1148-8"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2017.04.043"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.05.178"},{"key":"e_1_2_7_22_1","first-page":"5261","article-title":"Zein\u2010based nanocarriers as potential natural alternatives for drug and gene delivery: focus on cancer therapy","volume":"23","author":"Elzoghby A.","year":"2017","journal-title":"Curr. 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