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Consequently, finding newer anti\u2010retroviral pharmaceuticals by using biocompatible, biodegradable nanomaterials comprising a nanoparticle as core and a therapeutic agent is of high global interest. In this experiment, a second generation of a negatively charged nano\u2010biopolymer linear globular G2 dendrimer was carefully conjugated and loaded with well\u2010known anti\u2010HIV drugs lamivudine and efavirenz, respectively. They were characterised by a variety of analytical methods such as Zetasizer, Fourier\u2010transform infrared spectroscopy, elemental analysis and liquid chromatography\u2010mass spectroscopy. Additionally, conjugated lamivudine and loaded efazirenz with globular PEGylated G2 dendrimer were tested on an HEK293\u00a0T cell infected by single\u2010cycle replicable HIV\u20101 virion and evaluated using XTT test and HIV\u20101 P24 protein load. The results showed that lamivudine\u2010conjugated G2 significantly decreased retroviral activity without any cell toxicity. This effect was more or less observed by efavirenz\u2010loaded G2. These nano\u2010constructs are strongly suggested for further in vivo anti\u2010HIV assays.<\/jats:p>","DOI":"10.1049\/nbt2.12060","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T01:34:09Z","timestamp":1623375249000},"page":"627-637","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Lamivudine\u2010conjugated and efavirenz\u2010loaded G2 dendrimers: Novel anti\u2010retroviral nano drug delivery systems"],"prefix":"10.1049","volume":"15","author":[{"given":"Esmaeel Mohammadi","family":"Pargoo","sequence":"first","affiliation":[{"name":"Department of Biology, Science and Research Branch Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Reza","family":"Aghasadeghi","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazem","family":"Parivar","sequence":"additional","affiliation":[{"name":"Department of Biology, Science and Research Branch Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehri","family":"Nikbin","sequence":"additional","affiliation":[{"name":"Middle East Liver Diseases (MELD) Center  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8829-5373","authenticated-orcid":false,"given":"Pooneh","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Department of Hepatitis and AIDS Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi Shafiee","family":"Ardestani","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"265","published-online":{"date-parts":[[2021,6,10]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"HIV\/AIDS Fact Sheet","author":"WHO","year":"2018"},{"key":"e_1_2_7_3_1","unstructured":"UNAIDS:How AIDS changed everything\u2014MDG6: 15 years 15 lessons of hope from the AIDS response.http:\/\/www.unaids.org\/en\/resources\/documents\/2015\/MDG6_15years\u201015lessonsfromtheAIDSresponse. 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