{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:30:58Z","timestamp":1788226258541,"version":"build-2803163510"},"reference-count":49,"publisher":"Institution of Engineering and Technology (IET)","issue":"2","license":[{"start":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T00:00:00Z","timestamp":1641859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016173","name":"Rajshahi University","doi-asserted-by":"publisher","award":["A\u2010930\/5\/52\/RU\/Science\u201030\/20\u201021"],"award-info":[{"award-number":["A\u2010930\/5\/52\/RU\/Science\u201030\/20\u201021"]}],"id":[{"id":"10.13039\/501100016173","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In the present study, Ag\/AgCl\u2010NPs were biosynthesised using\n                    <jats:italic>Hypnea musciformis<\/jats:italic>\n                    seaweed extract; NPs synthesis was confirmed by a change of colour and observation of a razor\u2010sharp peak at 424\u00a0nm by UV\u2013visible spectroscopy. Synthesised nanoparticles were characterised by transmission electron microscopy, energy\u2010dispersive X\u2010ray spectroscopy, X\u2010ray powder diffraction and Fourier transform infrared spectroscopy. Bacterial cell growth inhibition proves that the Ag\/AgCl\u2010NPs have strong antibacterial activity and cell morphological alteration was observed in treated bacterial cells using propidium iodide (PI). Ag\/AgCl\u2010NPs inhibited Ehrlich ascites carcinoma (EAC) cells, colorectal cancer (HCT\u2010116) and breast cancer (MCF\u20107) cell line\n                    <jats:italic>in vitro<\/jats:italic>\n                    with the IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 40.45, 24.08 and 36.95\u00a0\u03bcg\/ml, respectively. Initiation of apoptosis in HCT\u2010116 and MCF\u20107 cells was confirmed using PI, FITC\u2010annexin V and Hoechst 33342 dye. No reaction oxygen species generation was observed in both treated and untreated cell lines. A significant increase of ATG\u20105 gene expression indicates the possibility of autophagy cell death besides apoptosis in MCF\u20107 cells. The initiation of apoptosis in EAC cells was confirmed by observing caspase\u20103 protein expression. Ag\/AgCl\u2010NPs inhibited 22.83% and 51% of the EAC cell growth\n                    <jats:italic>in vivo<\/jats:italic>\n                    in mice when administered 1.5 and 3.0\u00a0mg\/kg\/day (i.p.), respectively, for 5 consequent days.\n                  <\/jats:p>","DOI":"10.1049\/nbt2.12075","type":"journal-article","created":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T13:20:51Z","timestamp":1641907251000},"page":"49-60","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>Hypnea musciformis<\/i>\n                    \u2010mediated Ag\/AgCl\u2010NPs inhibit pathogenic bacteria, HCT\u2010116 and MCF\u20107 cells\u2019 growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice"],"prefix":"10.1049","volume":"16","author":[{"given":"Rita","family":"Ghose","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Faculty of Science University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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