{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T01:18:58Z","timestamp":1769822338003,"version":"3.49.0"},"reference-count":30,"publisher":"Institution of Engineering and Technology (IET)","issue":"9","license":[{"start":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T00:00:00Z","timestamp":1665014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005725","name":"National Plan for Science, Technology and Innovation","doi-asserted-by":"publisher","award":["15\u2010BIO4955\u201002"],"award-info":[{"award-number":["15\u2010BIO4955\u201002"]}],"id":[{"id":"10.13039\/501100005725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human pluripotent stem cells (hPSCs) can be proliferated on completely synthetic materials under xeno\u2010free cultivation conditions using biomaterials grafted with extracellular matrix protein (ECM)\u2010derived peptides. However, cell culture biomaterials grafted with ECM\u2010derived peptides must be prepared using a high concentration of peptide reaction solution (e.g. 1000\u00a0\u03bcg\/ml), whereas the ECM concentration of the ECM\u2010coated surface for hPSC culture is typically 5\u00a0\u03bcg\/ml. We designed a polyethylene glycol (PEG) joint nanosegment (linker) to be used between base cell culture biomaterials and bioactive ECM\u2010derived peptides to enhance the probability of contact between ECM\u2010derived peptides and cell binding receptors of hPSCs. Vitronectin\u2010derived peptides with glycine joint nanosegments (GCGG) were conjugated onto poly (vinyl alcohol\u2010co\u2010itaconic acid) hydrogels via PEG joint nanosegments, and human embryonic stem cells (hESCs) were cultivated on these hydrogels. hESCs could successfully be cultivated on hydrogels while maintaining their pluripotency and differentiation potential to differentiate into cells that are induced from three germ layers in vitro and in vivo, where only a 50\u00a0\u03bcg\/ml ECM\u2010derived peptide concentration was used when the PEG joint nanosegments were introduced into peptides that were grafted onto hydrogel surfaces. The joint nanosegments between bioactive peptides and base cell culture biomaterials were found to contribute to efficient hESC attachment and proliferation.<\/jats:p>","DOI":"10.1049\/nbt2.12091","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T09:20:16Z","timestamp":1665048016000},"page":"295-304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein\u2010derived peptides with polyethylene glycol joint nanosegments"],"prefix":"10.1049","volume":"16","author":[{"given":"Abdullah A.","family":"Alarfaj","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdurahman H.","family":"Hirad","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murugan A.","family":"Munusamy","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Suresh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Bharath Institute of Higher Education and Research  Chennai\u201073 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2970-8531","authenticated-orcid":false,"given":"Akon","family":"Higuchi","sequence":"additional","affiliation":[{"name":"Department of Chemical and Materials Engineering National Central University  Taoyuan Taiwan"},{"name":"Department of Reproduction National Center for Child Health and Development  Tokyo Japan"},{"name":"School of Ophthalmology and Optometry The Eye Hospital of Wenzhou Medical University Wenzhou Medical University  Wenzhou Zhejiang China"},{"name":"R&amp;D Center for Membrane Technology Chung Yuan Christian University  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"265","published-online":{"date-parts":[[2022,10,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c9bm00418a"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1116\/1.3525804"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.07.037"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136350"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010015\u20100005\u20104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4195"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep41165"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9704945"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.06.054"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/d1tb01878g"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/d1tb01555a"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201705546"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010020\u201001766\u20109"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2018.07.072"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pola.29353"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.6b01748"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45146"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409525111"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18136"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5b00188"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1629"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1532"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2013.07.017"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.01.015"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.09.006"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaz5894"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.10.039"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.119411"},{"key":"e_1_2_9_30_1","first-page":"57314","article-title":"Human 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