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Therefore, the authors aimed to utilise the characteristics of prodrug and nanotechnology to prepare a reactive oxygen species (ROS) and GSH dual\u2010responsive targeted tumour prodrug nanoparticle Man\u2010PEG\u2010SS\u2010PLGA\/ProPTX to improve the clinical application status of paclitaxel limitation. The characterisation of Man\u2010PEG\u2010SS\u2010PLGA\/ProPTX was carried out through preparation. The cytotoxicity of nanoparticles on tumour cells and the effect on apoptosis of tumour cells were investigated by cytotoxicity assay and flow cytometry analysis. The ROS responsiveness of nanoparticles was investigated by detecting the ROS level of tumour cells. The tumour cell selectivity of the nanoparticles was further investigated by receptor affinity assay and cell uptake assay. The particle size of Man\u2010PEG\u2010SS\u2010PLGA\/ProPTX was (132.90\u00a0\u00b1\u00a01.81) nm, the dispersion coefficient Polymer dispersity index was 0.13\u00a0\u00b1\u00a00.03, and the Zeta potential was (\u22128.65\u00a0\u00b1\u00a00.50) mV. The encapsulation rate was 95.46\u00a0\u00b1\u00a02.31% and the drug load was 13.65\u00a0\u00b1\u00a02.31%. The nanoparticles could significantly inhibit the proliferation and promote apoptosis of MCF\u20107, HepG2, and MDA\u2010MB\u2010231 tumour cells. It has good ROS response characteristics and targeting. The targeted uptake mechanism is energy\u2010dependent and endocytosis is mediated by non\u2010clathrin, non\u2010caveolin, lipid raft\/caveolin, and cyclooxygenase (COX)\/caveolin with a certain concentration dependence and time dependence. Man\u2010PEG\u2010SS\u2010PLGA\/ProPTX is a tumour microenvironment\u2010responsive nanoparticle that can actively target tumour cells. It restricts the release of PTX in normal tissues, enhances its selectivity to tumour cells, and has significant antitumour activity, which is expected to solve the current limitations of PTX use.<\/jats:p>","DOI":"10.1049\/nbt2.12119","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T22:00:43Z","timestamp":1681423243000},"page":"406-419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A paclitaxel prodrug nanoparticles with glutathion\/reactive oxygen species dual\u2010responsive and CD206 targeting to improve the anti\u2010tumour effect"],"prefix":"10.1049","volume":"17","author":[{"given":"Changhai","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of 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