{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:08:18Z","timestamp":1790183298988,"version":"4.1.0"},"reference-count":32,"publisher":"Institution of Engineering and Technology (IET)","issue":"4","license":[{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173989"],"award-info":[{"award-number":["82173989"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Nanobiotechnology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Dermaseptin\u2010PP is a newly discovered anticancer peptide with a unique antitumour mechanism and remarkable effect. However, this \u03b1\u2010helix anticancer peptide risks haemolysis when used at high doses, which limits its further application. This study aims to prepare a pH\u2010responsive liposome, Der\u2010loaded\u2010pHSL, using nanotechnology to avoid the haemolysis risk of Dermaseptin\u2010PP and increase its accumulation in tumour sites to enhance efficacy and reduce toxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The characterisation of Der\u2010loaded\u2010pHSL was carried out employing preparation. The effect of haemolysis and tumour inhibition were investigated by in vitro haemolysis assay and cytotoxicity assay. The cell uptake under different pH conditions was investigated by flow cytometry, and the effect of pH on tumour cell selectivity was evaluated. In order to evaluate the in vivo targeting and antitumour effect of Der\u2010loaded\u2010pHSL, the in vivo distribution experiment and the pharmacodynamic experiment were performed using the nude mouse tumour model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The preparation method of the Der\u2010loaded\u2010pHSL is simple, and the liposome has good nanoparticle characteristics. When Dermaseptin\u2010PP was prepared as liposome, haemolysis was significantly decreased, and tumour cell inhibition was significantly enhanced. Compared with ordinary liposomes, this change was more significant in Der\u2010loaded\u2010pHSL. The uptake of pH\u2010sensitive liposomes was higher in the simulated acidic tumour microenvironment, and the uptake showed a specific acid dependence. In vivo experiments showed that Der\u2010loaded\u2010pHSL had a significant tumour\u2010targeting effect and could significantly enhance the antitumour effect of Dermaseptin\u2010PP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Der\u2010loaded\u2010pHSL designed in this study is a liposome with a quick, simple, effective preparation method, which can significantly reduce the haemolytic toxicity of Dermaseptin\u2010PP and enhance its antitumour effect by increasing the tumour accumulation and cell intake. It provides a new idea for applying Dermaseptin\u2010PP and other anticancer peptides with \u03b1\u2010helical structure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1049\/nbt2.12128","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T02:42:30Z","timestamp":1681267350000},"page":"352-359","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Stimulus\u2010responsive nano lipidosome for enhancing the anti\u2010tumour effect of a novel peptide Dermaseptin\u2010PP"],"prefix":"10.1049","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1641-3814","authenticated-orcid":false,"given":"Changhai","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxue","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tangthianchaichana","family":"Jakkree","sequence":"additional","affiliation":[{"name":"Thammasat University  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouying","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Beijing University of Chinese Medicine  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"265","published-online":{"date-parts":[[2023,4,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.32802"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.jomfp_113_16"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010021\u201000572\u2010w"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275\u2010017\u20106452\u20101"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986732709200327085156"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2301.01005"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/ijn.s385470"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01421"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14102014"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516679973"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22101805"},{"issue":"7","key":"e_1_2_11_13_1","first-page":"3919","article-title":"The antimicrobial peptides and their potential clinical applications","volume":"11","author":"Lei J.","year":"2019","journal-title":"Am. 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