{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:11:08Z","timestamp":1787065868240,"version":"build-2736575974"},"reference-count":31,"publisher":"Institution of Engineering and Technology (IET)","issue":"5","license":[{"start":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T00:00:00Z","timestamp":1690588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Systems Biology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Meningiomas are common primary brain tumours, with macrophages playing a crucial role in their development and progression. This study aims to identify module genes correlated with meningioma\u2010associated macrophages and analyse their correlation with clinical features and immune infiltration.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We analysed single\u2010cell RNA sequencing (scRNA\u2010seq) data from two paired meningioma and normal meninges to identify meningioma\u2010associated macrophages. High\u2010dimensional weighted gene co\u2010expression network analysis (hdWGCNA) was employed to identify module genes linked to these macrophages, followed by functional enrichment and pseudotime trajectory analyses. A machine learning\u2010based model using the module genes was developed to predict tumour grades. Finally, meningiomas were classified into two molecular subtypes based on the module genes, followed by a comparison of clinical characteristics and immune cell infiltration.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Meningiomas exhibited a significantly higher proportion of macrophages than normal meninges, including novel macrophage clusters referred to as meningioma\u2010associated macrophages. The hdWGCNA analysis of macrophages within meningiomas unveiled 12 distinct modules, with the blue, black, and turquoise modules closely correlated with the meningioma\u2010associated macrophages. Hub genes within these modules were enriched in immune regulation, cellular communication, and metabolism pathways. Machine learning analysis identified 13 module genes (RSBN1, TIPRL, ATIC, SPP1, MALSU1, CDK1, MGP, DDIT3, SUPT16H, NFKBIA, SRSF5, ATXN2L, and UBB) strongly correlated with meningioma grade and constructed a predictive model with high accuracy and robustness. Based on the module genes, meningiomas were classified into two subtypes with distinct clinical and tumour microenvironment characteristics.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Our findings provide insights into the molecular characteristics underlying macrophage infiltration in meningiomas. The molecular signatures of macrophages demonstrate correlations with clinical features and immune cell infiltration in meningiomas.<\/jats:p>\n <\/jats:sec>","DOI":"10.1049\/syb2.12074","type":"journal-article","created":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T03:06:08Z","timestamp":1690599968000},"page":"259-270","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Single\u2010cell RNA sequencing identifies macrophage signatures correlated with clinical features and tumour microenvironment in meningiomas"],"prefix":"10.1049","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1456-0119","authenticated-orcid":false,"given":"Xiaowei","family":"Zhang","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"265","published-online":{"date-parts":[[2023,7,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1545-1"},{"key":"e_1_2_10_3_1","author":"Qt O.","journal-title":"Primary Brain and Other Central Nervous System Tumors [J]"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuchi.2014.05.006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21693"},{"key":"e_1_2_10_6_1","volume-title":"Meningioma [M]. 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