{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T18:13:23Z","timestamp":1767032003931,"version":"3.48.0"},"reference-count":46,"publisher":"Institution of Engineering and Technology (IET)","issue":"1","license":[{"start":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:00:00Z","timestamp":1766966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Systems Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sarcopenia is an ageing\u2010related disease characterised primarily by skeletal muscle functional decline. Despite of fatty acid metabolism (FAM) affecting oxidative stress within muscle tissue, the key roles of critical genes linking FAM and sarcopenia are unclear. The GSE8479, GSE1428, and GSE136344 datasets were downloaded and intersected for identifying FAM\u2010related differentially expressed genes (FAMRDEGs) screened by enrichment analysis, LASSO regression, and Support Vector Machine (SVM) analyses. Cytoscape software was used for visualising mRNA\u2010transcription factor (TF) and mRNA\u2010miRNA networks. In addition, ROC curves of key genes were plotted to evaluate their diagnostic significance. A Fatty Acid Metabolism Score (FAM\u2010Score) was conducted and immune cell infiltration analysis was conducted. The qPCR assay was performed to analyse the levels of screened critical genes. A total of 109 FAMRDEGs were obtained, and the LASSO regression and SVM models screened 14 of these genes. The network included 7 key genes with 54 miRNAs and 9 hub genes with 102\u00a0TFs. There were 6 types of immune cell infiltration showing statistical significance. The\n                    <jats:italic>FABP3<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001),\n                    <jats:italic>PECR<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.01), and\n                    <jats:italic>OPN3<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001) mRNA expression markedly increased in sarcopenia versus control groups. In contrast, sarcopenia group showed remarkably reduced\n                    <jats:italic>PCTP<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001),\n                    <jats:italic>SREBF2<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001), and\n                    <jats:italic>PPARGC1A<\/jats:italic>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.05) levels. This study provides reference indicators for FAM\u2010associated auxiliary biomarkers of sarcopenia and preliminarily establishes effective machine learning models for further mechanistic exploration.\n                  <\/jats:p>","DOI":"10.1049\/syb2.70052","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T18:11:44Z","timestamp":1767031904000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring and Validating the Molecular Mechanisms Linking Fatty Acid Metabolism and Sarcopenia"],"prefix":"10.1049","volume":"20","author":[{"given":"Ruopeng","family":"Yang","sequence":"first","affiliation":[{"name":"Hubei University of Chinese Medicine  Wuhan China"},{"name":"Department of Orthopaedics Traditional Chinese and Western Medicine Hospital Hubei University of Chinese Medicine  Wuhan China"},{"name":"Department of Orthopaedics Wuhan No.1 Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Key Laboratory of Theory and Application Research of Liver and Kidney in Traditional Chinese Medicine (Hubei Province Hospital of Traditional Chinese Medicine) Affiliated Hospital of Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Key Laboratory of Theory and Application Research of Liver and Kidney in Traditional Chinese Medicine (Hubei Province Hospital of Traditional Chinese Medicine), Hubei Shizhen Laboratory Affiliated Hospital of Hubei University of Chinese Medicine  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5119-2344","authenticated-orcid":false,"given":"Ping","family":"Xia","sequence":"additional","affiliation":[{"name":"Hubei University of Chinese Medicine  Wuhan China"},{"name":"Department of Orthopaedics Wuhan No.4 Hospital  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"265","published-online":{"date-parts":[[2025,12,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010024\u201000550\u2010w"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12838"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13081"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2022.37.e146"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1363889"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010021\u201004400\u20105"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.10.021"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2021.106497"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adl0389"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1193"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000465"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00249.2004"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084205"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"issue":"4","key":"e_1_2_10_17_1","first-page":"383","article-title":"Principal Component Analysis (PCA)","author":"Salem K. 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