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Tumour immune microenvironment (TME) may serve as a breakthrough for therapy. Chemokines are responsible for the recruitment of diverse immune cells in TME. We identify the association between chemokines and T and NK cells. This study was conducted to screen the chemokine derived from T and NK cells in OC single\u2010cell RNA sequencing (scRNA\u2010seq) dataset was acquired from the GEO database. Total 64 genes were collected. The intersection of marker genes of T and NK cells and chemokine\u2010related genes was considered as key chemokine\u2010related genes. Then, cell\u2010cell communication analysis, pseudotime analysis and transcription factors (TFs)\u2013target genes regulatory network construction, were performed. Immunohistochemical staining experiments and wound healing assays are performed. The results found 21 distinct cell subgroups and 8 core cell types. Total 667 and 611 marker genes were identified in T and NK cells, respectively. Eight key chemokine\u2010related genes were found. Also, 49 TFs\u2010related to cell subtypes were obtained, and the TFs with the highest activation degree was SPI1. Eight key chemokine\u2010related genes were mainly distributed in T cells, NK cells and monocyte cells. The pseudotime trajectory shown that key chemokine\u2010related genes were highly expressed in T and NK cells in the later stage of differentiation. CCL5 exhibits differences in ovarian malignant tumour tissues compared with normal ovarian tissues. These findings provide novel insights for subsequent exploration in OC.<\/jats:p>","DOI":"10.1049\/syb2.70069","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T00:08:27Z","timestamp":1782518907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Chemokine\u2010Related Genes Derived From\u00a0T and NK Cells in the Tumour Microenvironment of\u00a0Ovarian Cancer Based on scRNA\u2010Seq"],"prefix":"10.1049","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0263-4309","authenticated-orcid":false,"given":"Liang","family":"Wang","sequence":"first","affiliation":[{"name":"Laboratory of Gynecologic Oncology Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics &amp; Gynecology and Pediatrics Fujian Medical University  Fuzhou Fujian China"},{"name":"Fujian Key Laboratory of Women and Children's Critical Diseases Research 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