{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T18:01:26Z","timestamp":1780941686948,"version":"3.54.1"},"reference-count":69,"publisher":"Institution of Engineering and Technology (IET)","issue":"1","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":158,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["22ZR1450000"],"award-info":[{"award-number":["22ZR1450000"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Systems Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Triple\u2010negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options and poor prognosis. Anoikis resistance contributes to tumour dissemination, metastasis and therapeutic resistance. However, anoikis\u2010related molecular signatures integrating single\u2010cell and bulk transcriptomic data for prognostic prediction in TNBC remain insufficiently characterised. Bulk RNA\u2010seq data from TCGA\u2013TNBC and GSE58812, together with single\u2010cell transcriptomic data, were analysed. Anoikis\u2010related genes were identified by WGCNA and single\u2010cell module scoring. A prognostic signature was constructed using Cox and LASSO regression. Immune features and immunotherapy\u2010related indicators were evaluated, and STC2 was validated by tissue microarray, immunohistochemistry and in vitro anoikis assays. An anoikis\u2010related gene signature (\n                    <jats:italic>ASS1<\/jats:italic>\n                    ,\n                    <jats:italic>COL18A1<\/jats:italic>\n                    ,\n                    <jats:italic>STC2<\/jats:italic>\n                    ,\n                    <jats:italic>DHX36<\/jats:italic>\n                    ,\n                    <jats:italic>S100B<\/jats:italic>\n                    and\n                    <jats:italic>TGFBI<\/jats:italic>\n                    ) was established. High\u2010risk patients exhibited poor overall survival, increased cancer\u2010associated fibroblast infiltration and reduced T\u2010cell infiltration. Additionally, high anoikis risk scores were associated with increased mutations frequencies of\n                    <jats:italic>PTEN<\/jats:italic>\n                    ,\n                    <jats:italic>PIK3CA<\/jats:italic>\n                    and\n                    <jats:italic>ZEB2<\/jats:italic>\n                    , along with lower tumour mutation burden, microsatellite instability and PD\u2010L1 levels. IHC staining confirmed that patients with low STC2 expression had poor prognosis. Furthermore, STC2 knockdown reduced anoikis\u2010related apoptotic rates in an MDA\u2010MB\u2010231\u2010based anoikis\u2010mimic model in vitro. This study established an anoikis\u2010related gene signature that may improve prognostic stratification and reflect immunotherapy\u2010related features in TNBC.\n                  <\/jats:p>","DOI":"10.1049\/syb2.70076","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:50:38Z","timestamp":1780941038000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Analysis Identifies an Anoikis\u2010Related Gene Signature for Predicting Prognosis in Patients With Triple\u2010Negative Breast Cancer"],"prefix":"10.1049","volume":"20","author":[{"given":"Bing","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Jining Medical University  Jining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Jining Medical University  Jining China"},{"name":"School of Clinical Medicine Jining Medical University  Jining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu\u2010Di","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Jining Medical University  Jining China"},{"name":"Department of Pathology Shandong Public Health Clinical Center  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Jining Medical University  Jining China"},{"name":"School of Clinical Medicine Jining Medical University  Jining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Jining Medical University  Jining 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