{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T21:05:30Z","timestamp":1784322330989,"version":"3.55.0"},"reference-count":70,"publisher":"Institution of Engineering and Technology (IET)","issue":"1","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":197,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100019035","name":"Science and Technology Program of Hubei Province","doi-asserted-by":"publisher","award":["2024BCB035"],"award-info":[{"award-number":["2024BCB035"]}],"id":[{"id":"10.13039\/501100019035","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ietresearch.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["IET Systems Biology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>To explore the hub genes and pathways associated with sarcopenia progression from the perspective of mitochondrial dysfunction and macrophage activation. The GEO datasets GSE8479, GSE1428 and GSE136344 were obtained from the GEO database. Mitochondria\u2010related genes (MRGs) and macrophage activation\u2010related genes (MARGs) were identified. Functional enrichment analyses, PPI analysis, diagnostic model construction, molecular subtyping and ssGSEA\u2010based immune enrichment analysis were performed. Key genes were further validated by RT\u2010qPCR. A total of 62 mitochondria\u2010 and macrophage activation\u2010related differentially expressed genes (M&amp;MARDEGs) were identified. Nine key genes (IGF1, FTL, MAPK8, MAPK9, TF, CREB1, FAS, VEGFA and NFKB1) were retained in the final diagnostic model. Immune enrichment analysis suggested distinct immune\u2010related transcriptional characteristics among different risk groups and molecular subtypes. RT\u2010qPCR validation demonstrated that IGF1, FAS and MAPK9 were significantly downregulated, whereas FTL, TF, CREB1 and NFKB1 were significantly upregulated in the sarcopenia model group. MAPK8 and VEGFA showed decreasing trends without statistical significance. Integrated transcriptomic analysis identified key genes potentially associated with sarcopenia through the intersection of mitochondrial dysfunction and macrophage activation. These findings provide insights into the molecular characteristics of sarcopenia and may offer candidate targets for future mechanistic and translational studies.<\/jats:p>","DOI":"10.1049\/syb2.70081","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T20:36:27Z","timestamp":1784320587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Mitochondria\u2010 and Macrophage Activation\u2010Related Hub Genes in Sarcopenia"],"prefix":"10.1049","volume":"20","author":[{"given":"Bangzhi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Spine Surgery Traditional Chinese Medicine Hospital Affiliated to Chongqing Three Gorges Medical College  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Acupuncture and Orthopedics Hubei University of 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