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According to the European Food Safety Authority, 4786\u2009foodborne and waterborne outbreaks were reported in Europe in 2016, of which 0.4% were attributed to parasites including <jats:italic>Cryptosporidium<\/jats:italic>, <jats:italic>Giardia<\/jats:italic> and <jats:italic>Trichinella<\/jats:italic>. Until 2016, no standardized methods were available to detect <jats:italic>Giardia, Cryptosporidium<\/jats:italic> and <jats:italic>Toxoplasma<\/jats:italic> (oo)cysts in food. Therefore, no regulation exists regarding these biohazards. Nevertheless, considering their low infective dose, ingestion of foodstuffs contaminated by low quantities of these three parasites can lead to human infection. To evaluate the risk of protozoan parasites in food, efforts must be made towards exposure assessment to estimate the contamination along the food chain, from raw products to consumers. This requires determining: (i) the occurrence of infective protozoan (oo)cysts in foods, and (ii) the efficacy of control measures to eliminate this contamination. In order to conduct such assessments, methods for identification of viable (i.e. live) and infective parasites are required. This review describes the methods currently available to evaluate infectivity and viability of <jats:italic>G. duodenalis<\/jats:italic> cysts<jats:italic>, Cryptosporidium<\/jats:italic> spp. and <jats:italic>T. gondii<\/jats:italic> oocysts, and their potential for application in exposure assessment to determine the presence of the infective protozoa and\/or to characterize the efficacy of control measures. Advantages and limits of each method are highlighted and an analytical strategy is proposed to assess exposure to these protozoa.<\/jats:p>","DOI":"10.1051\/parasite\/2018009","type":"journal-article","created":{"date-parts":[[2018,3,19]],"date-time":"2018-03-19T08:49:55Z","timestamp":1521449395000},"page":"14","source":"Crossref","is-referenced-by-count":110,"title":["Assessing viability and infectivity of foodborne and waterborne stages (cysts\/oocysts) of <i>Giardia duodenalis, Cryptosporidium<\/i> spp., and <i>Toxoplasma gondii<\/i>: a review of methods"],"prefix":"10.1051","volume":"25","author":[{"given":"Ang\u00e9lique","family":"Rousseau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"La Carbona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lien","family":"Dum\u00e8tre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy J.","family":"Robertson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Gargala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandie","family":"Escotte-Binet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Favennec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Villena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9dric","family":"G\u00e9rard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Aubert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2018,3,19]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1128\/AEM.63.1.324-328.1997","volume":"63","author":"Abbaszadegan","year":"1997","journal-title":"Applied and Environmental Microbiology"},{"key":"R2","first-page":"476142","volume":"2014","author":"Adell","year":"2014","journal-title":"BioMed Research International,"},{"key":"R3","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/lam.12157","volume":"58","author":"Agull\u00f3-Barcel\u00f3","year":"2014","journal-title":"Letters in Applied Microbiology"},{"key":"R4","doi-asserted-by":"crossref","unstructured":"Aksoy U, Marangi M, Papini R, Ozkoc S, Bayram Delibas S, Giangaspero A. 2014. 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