{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:53:08Z","timestamp":1788886388859,"version":"build-2803163510"},"reference-count":19,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T00:00:00Z","timestamp":1527465600000},"content-version":"vor","delay-in-days":147,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2018,4,21]]},"published-print":{"date-parts":[[2018]]},"abstract":"\n A 28S rDNA PCR detection assay was previously developed to identify\n Dipylidium caninum<\/jats:italic>\n DNA inside single fleas collected from both cats and dogs. Sequence analysis of the 28S rDNA fragment indicated two genetically distinct variations of the target region. The two genotypes, so-called \u201c\n D.<\/jats:italic>\n \u2009\n caninum<\/jats:italic>\n canine genotype\u201d and \u201c\n D.<\/jats:italic>\n \u2009\n caninum<\/jats:italic>\n feline genotype\u201d, based on host origin, are further investigated and described in this paper. Restriction fragment length polymorphism (RFLP) analysis and hydrolysis probe-based genotyping assays were developed and validated for genotyping\n D.<\/jats:italic>\n \u2009\n caninum<\/jats:italic>\n DNA. The complete mitochondrial (mt) genome of the \u201cfeline genotype\u201d was sequenced and compared to the\n D.<\/jats:italic>\n \u2009\n caninum<\/jats:italic>\n mt genome available in GenBank. The molecular characterization of\n D.<\/jats:italic>\n \u2009\n caninum<\/jats:italic>\n isolates collected from infected fleas, and also proglottids collected from dogs and cats, confirmed the existence of two distinct genotypes. These genotypes are related to host origin (dogs or cats), irrespective of their geographical origin, and they present a biological adaptation to their respective host, as confirmed by the comparison of biological development and host preference in another study. The genetic differences (Part 1, present paper) and biological observations (Part 2, in this journal) enabled us to suggest the existence of two distinct species within\n D.<\/jats:italic>\n \u2009\n caninum<\/jats:italic>\n , which will have to be clarified.\n <\/jats:p>","DOI":"10.1051\/parasite\/2018028","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T03:59:55Z","timestamp":1527479995000},"page":"30","source":"Crossref","is-referenced-by-count":43,"title":["Analysis of\n Dipylidium caninum<\/i>\n tapeworms from dogs and cats, or their respective fleas"],"prefix":"10.1051","volume":"25","author":[{"given":"Michel","family":"Labuschagne","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Beugnet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Rehbein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Guillot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephus","family":"Fourie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionne","family":"Crafford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2018,5,28]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1017\/S0031182000058017","volume":"95","author":"Allsop","year":"1987","journal-title":"Parasitology"},{"issue":"(2)","key":"R2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.ympev.2012.08.023","volume":"69","author":"Bernt","year":"2013","journal-title":"Molecular Phylogenetics and Evolution"},{"key":"R3","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.vetpar.2014.06.008","volume":"205","author":"Beugnet","year":"2014","journal-title":"Veterinary Parasitology"},{"key":"R4","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1051\/parasite\/2018029","volume":"25","author":"Beugnet","year":"2018","journal-title":"Parasite"},{"key":"R5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.vetpar.2016.12.017","volume":"234","author":"Chandra","year":"2017","journal-title":"Veterinary Parasitology"},{"issue":"(2)","key":"R6","first-page":"109","volume":"10","author":"Crafford","year":"2013","journal-title":"LitNet Akademies"},{"key":"R7","first-page":"257","volume":"2","author":"East","year":"2013","journal-title":"International Journal for Parasitology: Parasites and Wildlife"},{"key":"R8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.vetpar.2009.07.041","volume":"10","author":"Gates","year":"2009","journal-title":"Veterinary Parasitology"},{"key":"R9","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1186\/s13071-016-1769-x","volume":"9","author":"Guo","year":"2016","journal-title":"Parasites & Vectors"},{"key":"R10","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.vetpar.2014.05.038","volume":"204","author":"Lahmar","year":"2014","journal-title":"Veterinary Parasitology,"},{"key":"R11","unstructured":"Littlewood DTJ, \nOlson PD. \n2001. Small subunit rDNA and the phylum Platyhelminthes: signal, noise, conflict and compromise, in \nInterrelationships of the Platyhelminthes, \nLittlewood DTJ, \nBray RA, Editors. \nTaylor and Francis: \nLondon. 262-278."},{"key":"R12","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1023\/A:1006219625792","volume":"45","author":"Loos-Frank","year":"2000","journal-title":"Systematic Parasitology"},{"key":"R13","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.vetpar.2017.08.015","volume":"245","author":"Low","year":"2017","journal-title":"Veterinary Parasitology"},{"key":"R14","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.ijpara.2012.11.014","volume":"43","author":"Nakao","year":"2013","journal-title":"International Journal for Parasitology"},{"key":"R15","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1016\/j.transproceed.2015.05.011","volume":"47","author":"Sahin","year":"2015","journal-title":"Transplantation Proceedings"},{"key":"R16","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1749-6632.1937.tb55485.x","volume":"37","author":"Venard","year":"1938","journal-title":"Annals of the New York Academy of Science"},{"issue":"(1)","key":"R17","first-page":"3","volume":"36","author":"Verster","year":"1969","journal-title":"Journal of Veterinary Research,"},{"key":"R18","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/BF02119999","volume":"4","author":"Witenberg","year":"1932","journal-title":"Zeitschrift f\u00fcr Parasitenkunde"},{"key":"R19","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.mcp.2007.05.003","volume":"21","author":"Zhang","year":"2007","journal-title":"Molecular and Cellular Probes"}],"container-title":["Parasite"],"original-title":[],"link":[{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2018028\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T04:06:22Z","timestamp":1584677182000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2018028"}},"subtitle":["Part 1. Molecular characterization of\n Dipylidium caninum<\/i>\n : genetic analysis supporting two distinct species adapted to dogs and cats"],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":19,"alternative-id":["parasite180011"],"URL":"https:\/\/doi.org\/10.1051\/parasite\/2018028","relation":{},"ISSN":["1776-1042"],"issn-type":[{"value":"1776-1042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}