{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:31:44Z","timestamp":1783384304436,"version":"3.54.6"},"reference-count":41,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T00:00:00Z","timestamp":1532304000000},"content-version":"vor","delay-in-days":203,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2018,7,7]]},"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic>causes one of the most common protozoal diseases of humans and animals worldwide. With the aim of designing an effective vaccine against<jats:italic>T. gondii<\/jats:italic>infection, we examined the immunogenicity of a DNA vaccine expressing heat shock protein 40 (HSP40) against challenge with<jats:italic>T. gondii<\/jats:italic>(type I RH and type II Pru) strains in Kunming mice. The plasmid pVAX1-HSP40 was constructed and used to immunize mice by intramuscular injection for three sequential immunizations with two-week intervals. This immunization regimen significantly reduced parasite cyst burden in pVAX1-HSP40-immunized mice (1871.9\u00a0\u00b1\u00a0142.3) compared with control mouse groups immunized with pVAX1 (3479.2\u00a0\u00b1\u00a0204.4), phosphate buffered saline (3024.4\u00a0\u00b1\u00a0212.8), or left untreated (3275.0\u00a0\u00b1\u00a0179.8) as healthy controls (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01). However, immunization failed to protect mice against challenge with the virulent RH strain. There was a significant increase in T lymphocyte subclasses (CD3e<jats:sup>+<\/jats:sup>CD4<jats:sup>+<\/jats:sup>T and CD3e<jats:sup>+<\/jats:sup>CD8a<jats:sup>+<\/jats:sup>T lymphocytes) in splenic tissues in immunized mice compared with controls (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). However, the level of antibodies, lymphocyte proliferation and concentration of cytokines (IFN-<jats:italic>\u03b3<\/jats:italic>, IL-2, IL-4, IL-10 and IL-12p70) were not significantly different between immunized and control mouse groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). These data indicate that pVAX1-HSP40 induced specific immune responses and achieved a significant reduction in the number of brain cysts in Pru-infected mice, and thus can be tested in future immunization studies along with plasmids containing other immunogenic proteins as a cocktail vaccine to fully abolish chronic toxoplasmosis.<\/jats:p>","DOI":"10.1051\/parasite\/2018040","type":"journal-article","created":{"date-parts":[[2018,7,24]],"date-time":"2018-07-24T07:54:40Z","timestamp":1532418880000},"page":"37","source":"Crossref","is-referenced-by-count":8,"title":["Immunization with plasmid DNA expressing Heat Shock Protein 40 confers prophylactic protection against chronic<i>Toxoplasma gondii<\/i>infection in Kunming mice"],"prefix":"10.1051","volume":"25","author":[{"given":"Zhong-Yuan","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian-Zhang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany 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