{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T22:40:19Z","timestamp":1784932819618,"version":"3.55.0"},"reference-count":32,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T00:00:00Z","timestamp":1536019200000},"content-version":"vor","delay-in-days":246,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2018,8,3]]},"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>\n\t\t\t\t\t<jats:italic>Giardia duodenalis<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> spp. are common human and animal pathogens. They have increasingly been reported in dairy calves in recent years; however, multilocus genotyping information for <jats:italic>G. duodenalis<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> infecting pre-weaned dairy calves in southwestern China is limited. In the present study, the prevalence of <jats:italic>G. duodenalis<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> spp. in pre-weaned dairy calves in central Sichuan province was determined and the pathogens were analyzed molecularly. Of 278 fecal samples from pre-weaned dairy calves, 26 (9.4%) were positive for <jats:italic>G. duodenalis<\/jats:italic> and 40 (14.4%) were positive for <jats:italic>Cryptosporidium<\/jats:italic> spp. <jats:italic>Cryptosporidium bovis<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a028), <jats:italic>Cryptosporidium ryanae<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a05) and <jats:italic>Cryptosporidium parvum<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a07) were detected. All seven <jats:italic>C. parvum<\/jats:italic> isolates were successfully subtyped based on the <jats:italic>gp60<\/jats:italic> gene sequence, and only IIdA15G1 was detected. Multilocus sequence typing of <jats:italic>G. duodenalis<\/jats:italic> based on beta-giardin (<jats:italic>bg<\/jats:italic>), triose phosphate isomerase (<jats:italic>tpi<\/jats:italic>) and glutamate dehydrogenase (<jats:italic>gdh<\/jats:italic>) genes revealed 19 different assemblage E multilocus genotypes (two known and 17 unpublished genotypes). Based on eBURST analysis, a high degree of genetic diversity within assemblage E was observed in pre-weaned dairy calves in Sichuan province. To the best of our knowledge, this is the first study using multilocus sequence typing and eBURST analysis to characterize <jats:italic>G. duodenalis<\/jats:italic> in pre-weaned dairy calves in southwestern China.<\/jats:p>","DOI":"10.1051\/parasite\/2018046","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T09:49:16Z","timestamp":1536054556000},"page":"45","source":"Crossref","is-referenced-by-count":37,"title":["Occurrence and genotyping of <i>Giardia duodenalis<\/i> and <i>Cryptosporidium<\/i> in pre-weaned dairy calves in central Sichuan province, China"],"prefix":"10.1051","volume":"25","author":[{"given":"Zhijun","family":"Zhong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Dan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangwen","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinan","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suizhong","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junliang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobin","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangneng","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2018,9,4]]},"reference":[{"issue":"13","key":"R1","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1016\/j.ijpara.2008.04.008","volume":"38","author":"Caccio","year":"2008","journal-title":"International 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