{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:01:23Z","timestamp":1791388883371,"version":"4.3.3"},"reference-count":25,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T00:00:00Z","timestamp":1537228800000},"content-version":"vor","delay-in-days":260,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2018,8,6]]},"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Although microscopic examination of stool samples remains the reference method for the diagnosis of intestinal protozoal infections, these techniques are time-consuming and require operators who are experienced and well trained. Molecular biology seems to offer performances at least equivalent in terms of sensitivity and specificity for certain parasites. This study aimed to compare three multiplex PCR assays on 93 prospectively collected positive stools (prospective cohort) and a panel of 12 more <jats:italic>Cryptosporidium<\/jats:italic>-positive samples (<jats:italic>Cryptosporidium<\/jats:italic> panel). On the prospective cohort, the sensitivity was 89%, 64% and 41% for <jats:italic>Giardia<\/jats:italic> sp. detection for BD Max<jats:sup>TM<\/jats:sup>, G-DiaPara<jats:sup>TM<\/jats:sup> and RIDA<jats:sup>\u00ae<\/jats:sup>GENE, respectively and 75%, 100% and 100% for <jats:italic>C. parvum\/hominis<\/jats:italic> detection. The sensitivity of the RIDA<jats:sup>\u00ae<\/jats:sup>GENE assay for all <jats:italic>Cryptosporidium<\/jats:italic> species was 100%, and for <jats:italic>D. fragilis<\/jats:italic> 71%. All the techniques obtained the same results for <jats:italic>E. histolytica<\/jats:italic> detection, with one positive sample. All species in the <jats:italic>Cryptosporidium<\/jats:italic> panel were identified by the RIDA<jats:sup>\u00ae<\/jats:sup>GENE PCR. The BD Max<jats:sup>TM<\/jats:sup> and G-DiaPara<jats:sup>TM<\/jats:sup> assays detected only <jats:italic>C. parvum\/hominis<\/jats:italic> with the exception of one positive sample for <jats:italic>C. meleagridis<\/jats:italic>. No assay showed satisfactory results for all parasites simultaneously, and the DNA extraction seems to be the critical step. More studies are needed to standardize this procedure.<\/jats:p>","DOI":"10.1051\/parasite\/2018049","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T10:10:52Z","timestamp":1537351852000},"page":"48","source":"Crossref","is-referenced-by-count":43,"title":["Comparison of three commercial multiplex PCR assays for the diagnosis of intestinal protozoa"],"prefix":"10.1051","volume":"25","author":[{"given":"Brice","family":"Autier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorya","family":"Belaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romy","family":"Razakandrainibe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Gangneux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Robert-Gangneux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2018,9,18]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.mm6453a1","volume":"64","author":"Adams","year":"2017","journal-title":"MMWR, Morbidity and Mortality Weekly Report"},{"key":"R2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1051\/parasite\/2010174299","volume":"17","author":"Adamska","year":"2010","journal-title":"Parasite"},{"key":"R3","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1111\/tmi.12848","volume":"22","author":"Al-Areeqi","year":"2017","journal-title":"Tropical Medicine & International Health: TM & IH"},{"key":"R4","first-page":"19642","volume":"15","author":"ANOFEL Cryptosporidium National Network","year":"2010","journal-title":"Euro Surveillance: Bulletin Europ\u00e9en sur les Maladies Transmissibles\u00a0=\u00a0European Communicable Disease Bulletin"},{"key":"R5","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1051\/parasite\/1966416607","volume":"41","author":"Bailenger","year":"1966","journal-title":"Annales de Parasitologie Humaine et Compar\u00e9e"},{"key":"R6","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.vetpar.2016.07.025","volume":"227","author":"Chan","year":"2016","journal-title":"Veterinary Parasitology"},{"key":"R7","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1099\/jmm.0.066050-0","volume":"63","author":"Elwin","year":"2014","journal-title":"Journal of Medical Microbiology"},{"key":"R8","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0140-6736(14)61682-2","volume":"385","author":"GBD 2013 Mortality and Causes of Death Collaborators","year":"2015","journal-title":"Lancet"},{"key":"R9","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.diagmicrobio.2015.11.005","volume":"84","author":"Gotfred-Rasmussen","year":"2016","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"key":"R10","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1051\/parasite\/2013051","volume":"20","author":"Jeddi","year":"2013","journal-title":"Parasite"},{"key":"R11","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1017\/S0031182017000129","volume":"144","author":"Kaisar","year":"2017","journal-title":"Parasitology"},{"key":"R12","doi-asserted-by":"crossref","first-page":"190.e1","DOI":"10.1016\/j.cmi.2015.10.019","volume":"22","author":"Laude","year":"2016","journal-title":"Clinical Microbiology and Infection"},{"key":"R13","doi-asserted-by":"crossref","first-page":"e20","DOI":"10.1155\/2014\/152651","volume":"25","author":"Lloyd","year":"2014","journal-title":"Canadian Journal of Infectious Diseases & Medical Microbiology"},{"key":"R14","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.1128\/JCM.00765-16","volume":"54","author":"Madison-Antenucci","year":"2016","journal-title":"Journal of Clinical Microbiology"},{"key":"R15","unstructured":"Martens D, von Thien H, Tannich E. 2013. eP721. 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