{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:30:45Z","timestamp":1790141445876,"version":"4.0.1"},"reference-count":53,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":329,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2018,10,29]]},"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Cystic echinococcosis (CE) is a cosmopolitan parasitic disease caused by infection with the larval stage of <jats:italic>Echinococcus granulosus sensu lato<\/jats:italic>. Thioredoxin peroxidase (TPx) may play an essential role in the antioxidant defence system of <jats:italic>E. granulosus s.l.<\/jats:italic> as neither catalase nor glutathione peroxidase activities have been detected in the parasite. However, it is not known whether TPx affects the survival and growth of <jats:italic>E. granulosus s.l.<\/jats:italic> during development. In this study, three fragments of siRNA specific for <jats:italic>EgTPx<\/jats:italic> (siRNA-1\/2\/3) were designed and transfected into protoscoleces of <jats:italic>E. granulosus sensu stricto<\/jats:italic> by electroporation. Quantitative real-time PCR and Western blotting analysis showed that siRNA-3 significantly reduced the expression of <jats:italic>EgTPx.<\/jats:italic> Coincidentally, knockdown of <jats:italic>EgTPx<\/jats:italic> expression in protoscoleces with siRNA-3 significantly reduced the viability of the parasite under oxidative stress induced by 0.6\u00a0mM H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. <jats:italic>In vitro<\/jats:italic> culture studies showed that protoscoleces treated with siRNA-3 reduced pre-microcyst formation. <jats:italic>In vivo<\/jats:italic> experiments showed that injecting mice intraperitoneally with protoscoleces treated with siRNA-3 resulted in a significant reduction in the number, size and weight of CE cysts compared with those of control animals. Silencing of <jats:italic>EgTPx<\/jats:italic> led to the impairment of growth of <jats:italic>E. granulosus s.s.<\/jats:italic> both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, indicating that <jats:italic>EgTPx<\/jats:italic> is an important factor for protoscoleces survival and plays an important role in the antioxidant defence against the host during development.<\/jats:p>","DOI":"10.1051\/parasite\/2018055","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T09:14:19Z","timestamp":1543223659000},"page":"57","source":"Crossref","is-referenced-by-count":19,"title":["<i>Echinococcus granulosus sensu stricto<\/i>: silencing of thioredoxin peroxidase impairs the differentiation of protoscoleces into metacestodes"],"prefix":"10.1051","volume":"25","author":[{"given":"Hui","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanshan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoping","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"Peter McManus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2018,11,26]]},"reference":[{"issue":"3","key":"R1","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/JCM.02420-15","volume":"54","author":"Agudelo Higuita","year":"2016","journal-title":"Journal of Clinical Microbiology"},{"key":"R2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13071-014-0610-7","volume":"8","author":"Ahn","year":"2015","journal-title":"Parasites & Vectors"},{"issue":"Suppl 1","key":"R3","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s11259-009-9247-y","volume":"33","author":"Battelli","year":"2009","journal-title":"Veterinary Research Communications"},{"issue":"26","key":"R4","doi-asserted-by":"crossref","first-page":"17898","DOI":"10.1074\/jbc.M710609200","volume":"283","author":"Bonilla","year":"2008","journal-title":"Journal of Biological Chemistry"},{"issue":"2","key":"R5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0166-6851(03)00078-1","volume":"128","author":"Boyle","year":"2003","journal-title":"Molecular and Biochemical Parasitology"},{"issue":"1\u20132","key":"R6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.vetpar.2015.06.003","volume":"212","author":"Britton","year":"2015","journal-title":"Veterinary Parasitology"},{"issue":"8","key":"R7","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1002\/pmic.200300487","volume":"3","author":"Chemale","year":"2003","journal-title":"Proteomics"},{"issue":"2","key":"R8","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.molbiopara.2005.06.007","volume":"143","author":"Correnti","year":"2005","journal-title":"Molecular and Biochemical Parasitology"},{"issue":"6","key":"R9","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/S1473-3099(07)70134-2","volume":"7","author":"Craig","year":"2007","journal-title":"Lancet Infectious Diseases"},{"key":"R10","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.jprot.2013.04.013","volume":"84","author":"Cui","year":"2013","journal-title":"Journal of Proteomics"},{"key":"R11","unstructured":"Docampo R. 1995. 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