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It is in this context that the presence of <jats:italic>Wolbachia<\/jats:italic> and <jats:italic>Sodalis glossinidius<\/jats:italic> was studied in wild populations of <jats:italic>Glossina fuscipes quanzensis<\/jats:italic> from the Democratic Republic of Congo. Tsetse flies were captured with pyramidal traps. Of the 700 <jats:italic>Glossina f. quanzensis<\/jats:italic> captured, 360 were dissected and their midguts collected and analyzed. <jats:italic>Sodalis glossinidius<\/jats:italic> and <jats:italic>Wolbachia<\/jats:italic> were identified by PCR. The <jats:italic>Wolbachia-<\/jats:italic>positive samples were genetically characterized with five molecular markers. PCR revealed 84.78% and 15.55% midguts infected by <jats:italic>Wolbachia<\/jats:italic> and <jats:italic>S. glossinidius,<\/jats:italic> respectively. The infection rates varied according to capture sites. Of the five molecular markers used to characterize <jats:italic>Wolbachia<\/jats:italic>, only the fructose bis-phosphate aldolase gene was amplified for about 60% of midguts previously found with <jats:italic>Wolbachia<\/jats:italic> infections. The sequencing results confirmed the presence of <jats:italic>Wolbachia<\/jats:italic> and revealed the presence of <jats:italic>S. glossinidius<\/jats:italic> in the midgut of <jats:italic>Glossina f<\/jats:italic>. <jats:italic>quanzensis.<\/jats:italic> A low level of midguts were naturally co-infected by both bacteria. The data generated in this study open a framework for investigations aimed at understanding the contribution of these symbiotic microorganisms to the vectorial competence of <jats:italic>Glossina fuscipes quanzensis<\/jats:italic>.<\/jats:p>","DOI":"10.1051\/parasite\/2019005","type":"journal-article","created":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T08:55:29Z","timestamp":1549529729000},"page":"5","source":"Crossref","is-referenced-by-count":8,"title":["Molecular identification of <i>Wolbachia<\/i> and <i>Sodalis glossinidius<\/i> in the midgut of <i>Glossina fuscipes quanzensis<\/i> from the Democratic Republic of Congo"],"prefix":"10.1051","volume":"26","author":[{"given":"Gustave","family":"Simo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sartrien 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