{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:28:30Z","timestamp":1790411310148,"version":"4.1.0"},"reference-count":37,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":59,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2019,2,18]]},"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>Schistosomiasis is one of the most significant parasitic diseases of humans. The hybridization of closely related <jats:italic>Schistosoma<\/jats:italic> species has already been documented. However, hybridization between phylogenetically distant species is unusual. In the present study, we characterized the causative agent of schistosomiasis in a 14-year-old patient with hematuria from C\u00f4te d\u2019Ivoire, using morphological and molecular approaches. A 24-hour parasitological examination of urine showed the presence of numerous eggs (150\u00a0\u03bcm long\u00a0\u00d7\u00a062\u00a0\u03bcm wide) with a lateral spine (25\u00a0\u03bcm), identified morphologically as <jats:italic>Schistosoma mansoni<\/jats:italic>. Examination of stools performed on the same day found no parasites. The urine and stool examinations of the patient\u2019s family members performed two weeks later showed neither parasites nor hematuria; but in contrast, many <jats:italic>S. mansoni<\/jats:italic> eggs were found again in the patient\u2019s urine, but never in his stools. Conventional PCRs were performed, using two primer pairs targeting 28S-rDNA and COI mtDNA. The 28S-rDNA sequence of these eggs, compared with two reference sequences from GenBank demonstrated a hybrid with 25 double peaks, indicating clearly hybrid positions (5.37%) between <jats:italic>S.\u00a0mansoni<\/jats:italic> and <jats:italic>S.\u00a0haematobium.<\/jats:italic> Similarly, we identified a unique <jats:italic>S. mansoni<\/jats:italic> COI sequence for the two eggs, with 99.1% homology with the <jats:italic>S. mansoni<\/jats:italic> reference sequence. Consequently, this case was the result of hybridization between an <jats:italic>S.\u00a0haematobium<\/jats:italic> male and an <jats:italic>S. mansoni<\/jats:italic> female. This should be taken into consideration to explore the elimination of ectopic schistosome eggs in the future.<\/jats:p>","DOI":"10.1051\/parasite\/2019010","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T09:14:26Z","timestamp":1551431666000},"page":"10","source":"Crossref","is-referenced-by-count":18,"title":["No limit in interspecific hybridization in schistosomes: observation from a case report"],"prefix":"10.1051","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6995-0088","authenticated-orcid":false,"given":"J\u00e9r\u00f4me","family":"Depaquit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Akhoundi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djamel","family":"Haouchine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Mantelet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arezki","family":"Izri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2019,3,1]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1136\/jcp.23.6.545","volume":"23","author":"Allen","year":"1970","journal-title":"Journal of Clinical Pathology"},{"key":"R2","unstructured":"Belleli V. 1886. 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