{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T10:02:18Z","timestamp":1770890538977,"version":"3.50.1"},"reference-count":31,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"vor","delay-in-days":120,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2019,4,16]]},"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>To assess the prevalence of <jats:italic>Toxoplasma gondii<\/jats:italic> infection in native and commensal rodents as indicators of environmental pollution, we analyzed brain tissue from small mammals collected on legal and illegal waste sites in the Slovenian and Croatian parts of Istria. A total of 136 animals and five species of the family Muridae were analyzed: black rat (<jats:italic>Rattus rattus<\/jats:italic>), domestic mouse (<jats:italic>Mus musculus<\/jats:italic>), wood mouse (<jats:italic>Apodemus sylvaticus<\/jats:italic>), striped field mouse (<jats:italic>Apodemus agrarius<\/jats:italic>), and yellow-necked mouse (<jats:italic>Apodemus flavicollis<\/jats:italic>). Using quantitative Polymerase Chain Reaction (qPCR), <jats:italic>T. gondii<\/jats:italic> DNA was detected in four homogenized brain tissue samples (2.94%), from all of the analyzed species, except black rat. Out of these, two samples, domestic mouse (<jats:italic>Mus musculus<\/jats:italic>) and wood mouse (<jats:italic>Apodemus sylvaticus<\/jats:italic>) had sufficient DNA for genotyping of <jats:italic>T. gondii<\/jats:italic> isolates in which we demonstrated the presence of clonal type II using RFLP PCR with four markers (SAG1, SAG2, GRA6 and GRA7). Three of four infected animals (75%) were collected on dumpsites.<\/jats:p>","DOI":"10.1051\/parasite\/2019027","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T08:24:50Z","timestamp":1556699090000},"page":"27","source":"Crossref","is-referenced-by-count":15,"title":["Prevalence and genotype identification of <i>Toxoplasma gondii<\/i> in suburban rodents collected at waste disposal sites"],"prefix":"10.1051","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1562-9528","authenticated-orcid":false,"given":"Vladimir","family":"Ivovic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Potusek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Buzan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2019,5,1]]},"reference":[{"key":"R1","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1017\/S0950268899002113","volume":"122","author":"Aramini","year":"1999","journal-title":"Epidemiology and Infection"},{"key":"R2","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1098\/rspb.2000.1182","volume":"267","author":"Berdoy","year":"2000","journal-title":"Proceedings of the Royal Society of London"},{"issue":"12","key":"R3","doi-asserted-by":"crossref","first-page":"e28925","DOI":"10.1371\/journal.pone.0028925","volume":"6","author":"Berenreiterov\u00e1","year":"2011","journal-title":"PLoS One"},{"issue":"1","key":"R4","doi-asserted-by":"crossref","first-page":"573","DOI":"10.15666\/aeer\/1501_573588","volume":"15","author":"Buzan","year":"2017","journal-title":"Applied Ecology and Environmental Research: International Scientific Journal"},{"key":"R5","doi-asserted-by":"crossref","first-page":"723","DOI":"10.2307\/3283961","volume":"81","author":"Dubey","year":"1995","journal-title":"Journal of Parasitology"},{"key":"R6","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.ijpara.2009.01.005","volume":"39","author":"Dubey","year":"2009","journal-title":"International Journal for Parasitology"},{"key":"R7","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/s12985-017-0798-1","volume":"14","author":"Duh","year":"2017","journal-title":"Virology Journal"},{"key":"R8","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1186\/1756-3305-7-196","volume":"7","author":"Ferroglio","year":"2014","journal-title":"Parasites and Vectors"},{"issue":"3\u20134","key":"R9","first-page":"267","volume":"49","author":"Galal","year":"2018","journal-title":"International Journal of Parasitology"},{"key":"R10","unstructured":"Gilot-Fromont E, L\u00e9lu M, Dard\u00e9 ML, Richomme C, Aubert D, Afonso E, Mercier A, Gotteland C, Villena I. 2012. The life cycle of Toxoplasma gondii in the natural environment, in Toxoplasmosis \u2013 Recent Advances, Djurkovic-Djakovic O., Editor, InTech, p. 3\u201336."},{"issue":"1","key":"R11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","volume":"30","author":"Homan","year":"2000","journal-title":"International Journal for Parasitology"},{"key":"R12","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1128\/JCM.35.6.1411-1414.1997","volume":"35","author":"Howe","year":"1997","journal-title":"Journal of Clinical Microbiology"},{"key":"R13","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1093\/infdis\/172.6.1561","volume":"172","author":"Howe","year":"1995","journal-title":"Journal of Infectious Diseases"},{"issue":"3","key":"R14","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1515\/acve-2015-0029","volume":"65","author":"Ivovic","year":"2015","journal-title":"Acta Veterinaria"},{"key":"R15","unstructured":"Ivovi\u0107 V, Vujani\u0107 M, \u017divkovi\u0107 T, Klun I, Djurkovi\u0107-Djakovi\u0107 O. 2012. Molecular detection and genotyping of Toxoplasma gondii from clinical samples, in Toxoplasmosis - Recent Advances, Djurkovic-Djakovic O., Editor, InTech. p. 103\u2013120."},{"key":"R16","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1093\/aje\/154.4.357","volume":"154","author":"Jones","year":"2001","journal-title":"American Journal of Epidemiology"},{"key":"R17","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.vetpar.2008.05.030","volume":"156","author":"Kijlstra","year":"2008","journal-title":"Veterinary Parasitology"},{"key":"R18","first-page":"12","volume":"52","author":"Kokalj","year":"2005","journal-title":"Geografski Vestnik"},{"key":"R19","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1016\/j.resconrec.2008.07.003","volume":"52","author":"Mazzanti","year":"2008","journal-title":"Resources, Conservation and Recycling"},{"key":"R20","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.vetpar.2011.09.001","volume":"184","author":"Meerburg","year":"2012","journal-title":"Veterinary Parasitology"},{"key":"R21","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.rvsc.2005.09.007","volume":"81","author":"Pena","year":"2006","journal-title":"Research in Veterinary Science"},{"key":"R22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2334-3-7","volume":"3","author":"Reischl","year":"2003","journal-title":"BMC Infectious Diseases"},{"key":"R23","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1051\/parasite\/2014058","volume":"21","author":"Rend\u00f3n-Franco","year":"2014","journal-title":"Parasite"},{"key":"R24","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.pt.2008.10.004","volume":"25","author":"Rosenthal","year":"2009","journal-title":"Trends in Parasitology"},{"key":"R25","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1654-109X.2011.01175.x","volume":"15","author":"Rusterholz","year":"2012","journal-title":"Applied Vegetation Science"},{"key":"R26","doi-asserted-by":"crossref","first-page":"383859","DOI":"10.1155\/2014\/383859","volume":"2014","author":"Saki","year":"2014","journal-title":"BioMed Research International"},{"key":"R27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182009991065","volume":"137","author":"Su","year":"2010","journal-title":"Parasitology"},{"key":"R28","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","volume":"30","author":"Tenter","year":"2000","journal-title":"International Journal for Parasitology"},{"key":"R29","doi-asserted-by":"crossref","first-page":"4035","DOI":"10.1128\/AEM.70.7.4035-4039.2004","volume":"70","author":"Villena","year":"2004","journal-title":"Applied and Environmental Microbiology"},{"key":"R30","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1089\/vbz.2010.0119","volume":"11","author":"Vujani\u0107","year":"2011","journal-title":"Vector-Borne and Zoonotic Diseases"},{"key":"R31","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/B978-0-12-800182-0.00003-9","volume":"85","author":"Worth","year":"2014","journal-title":"Advances in Parasitology"}],"container-title":["Parasite"],"original-title":[],"link":[{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2019027\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T08:03:32Z","timestamp":1584691412000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.parasite-journal.org\/10.1051\/parasite\/2019027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":31,"alternative-id":["parasite190023"],"URL":"https:\/\/doi.org\/10.1051\/parasite\/2019027","relation":{},"ISSN":["1776-1042"],"issn-type":[{"value":"1776-1042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}