{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:51:19Z","timestamp":1773028279738,"version":"3.50.1"},"reference-count":25,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":41,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2020,1,20]]},"published-print":{"date-parts":[[2020]]},"abstract":"<jats:p>Eight microsatellite loci, recently developed in the species<jats:italic>Anisakis pegreffii,<\/jats:italic>were successfully amplified in<jats:italic>Anisakis berlandi<\/jats:italic>, sibling species of the<jats:italic>A. simplex<\/jats:italic>(s. l.) complex. They were validated on adult specimens (<jats:italic>n<\/jats:italic>\u00a0=\u00a046) of the parasite species, collected from two individuals of the definitive host, the long-finned pilot whale<jats:italic>Globicephala melas<\/jats:italic>from New Zealand waters. Among the eight loci scored, one,<jats:italic>Anisl 07132,<\/jats:italic>had null alleles in<jats:italic>A. berlandi<\/jats:italic>and was thus excluded from the subsequent genetic analysis. Two loci,<jats:italic>Anisl 00314<\/jats:italic>and<jats:italic>Anisl 10535,<\/jats:italic>were monomorphic. In addition, as also previously detected in the other species of the<jats:italic>A. simplex<\/jats:italic>(s. l.) complex, the<jats:italic>Anisl 7<\/jats:italic>locus was seen to be sex-linked, showing hemizygosity in male specimens. Differential allele frequency distributions of<jats:italic>A. berlandi,<\/jats:italic>with respect to those previously observed in<jats:italic>A. pegreffii<\/jats:italic>and<jats:italic>A. simplex<\/jats:italic>(s. s.), were found at some microsatellite loci. The<jats:italic>Anisl 7<\/jats:italic>locus provided 100% diagnosis between<jats:italic>A. berlandi<\/jats:italic>and<jats:italic>A. pegreffii,<\/jats:italic>while others resulted in 99% diagnosis between<jats:italic>A. berlandi<\/jats:italic>and the other two species. Simple sequence repeat (SSR) loci also allowed us to estimate the genetic differentiation of<jats:italic>A. berlandi<\/jats:italic>from<jats:italic>A. pegreffii<\/jats:italic>(<jats:italic>F<\/jats:italic><jats:sub>st<\/jats:sub>\u00a0\u2248\u00a00.45, Dc\u00a0=\u00a00.82) and<jats:italic>A. simplex<\/jats:italic>(s. s.) (<jats:italic>F<\/jats:italic><jats:sub>st<\/jats:sub>\u00a0\u2248\u00a00.57, Dc\u00a0=\u00a00.73). The results suggest that SSRs provide a set of candidate markers for population genetics analysis of<jats:italic>A. berlandi<\/jats:italic>, as well as for the investigation, through a multi-locus genotyping approach, of possible patterns of hybridisation\/introgression events between<jats:italic>A. berlandi<\/jats:italic>and the other two<jats:italic>Anisakis<\/jats:italic>species in sympatric conditions.<\/jats:p>","DOI":"10.1051\/parasite\/2020004","type":"journal-article","created":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T10:44:28Z","timestamp":1581417868000},"page":"9","source":"Crossref","is-referenced-by-count":19,"title":["Cross-species utility of microsatellite loci for the genetic characterisation of<i>Anisakis berlandi<\/i>(Nematoda: Anisakidae)"],"prefix":"10.1051","volume":"27","author":[{"given":"Eleonora","family":"Bello","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Paoletti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen C.","family":"Webb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Nascetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simonetta","family":"Mattiucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2020,2,11]]},"reference":[{"key":"R1","first-page":"233","volume":"19","author":"Cavalli-Sforza","year":"1967","journal-title":"American Journal of Human Genetics"},{"key":"R2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1755-0998.2010.02847.x","volume":"10","author":"Excoffier","year":"2010","journal-title":"Molecular Ecology Resources"},{"key":"R3","unstructured":"Felsenstein J. 1993. 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