{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:59:35Z","timestamp":1790690375291,"version":"4.1.0"},"reference-count":69,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":135,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2020,4,30]]},"published-print":{"date-parts":[[2020]]},"abstract":"<jats:p>Microsatellites can be utilized to explore genotypes, population structure, and other genomic features of eukaryotes<jats:italic>.<\/jats:italic> Systematic characterization of microsatellites has not been a focus for several species of <jats:italic>Plasmodium<\/jats:italic>, including <jats:italic>P. malariae and P. ovale,<\/jats:italic> as the majority of malaria elimination programs are focused on <jats:italic>P. falciparum<\/jats:italic> and to a lesser extent <jats:italic>P. vivax.<\/jats:italic> Here, five human malaria species (<jats:italic>P. falciparum<\/jats:italic>, <jats:italic>P. vivax<\/jats:italic>, <jats:italic>P. malariae<\/jats:italic>, <jats:italic>P. ovale curtisi<\/jats:italic>, and <jats:italic>P. knowlesi<\/jats:italic>) were investigated with the aim of conducting in-depth categorization of microsatellites for <jats:italic>P. malariae<\/jats:italic> and <jats:italic>P. ovale curtisi<\/jats:italic>. Investigation of reference genomes for microsatellites with unit motifs of 1\u201310 base pairs indicates high diversity among the five <jats:italic>Plasmodium<\/jats:italic> species. <jats:italic>Plasmodium malariae,<\/jats:italic> with the largest genome size, displays the second highest microsatellite density (1421 No.\/Mbp; 5% coverage) next to <jats:italic>P. falciparum<\/jats:italic> (3634 No.\/Mbp; 12% coverage). The lowest microsatellite density was observed in <jats:italic>P. vivax<\/jats:italic> (773 No.\/Mbp; 2% coverage). A, AT, and AAT are the most commonly repeated motifs in the <jats:italic>Plasmodium<\/jats:italic> species. For <jats:italic>P. malariae<\/jats:italic> and <jats:italic>P. ovale curtisi<\/jats:italic>, microsatellite-related sequences are observed in approximately 18\u201329% of coding sequences (CDS). Lysine, asparagine, and glutamic acids are most frequently coded by microsatellite-related CDS. The majority of these CDS could be related to the gene ontology terms \u201ccell parts,\u201d \u201cbinding,\u201d \u201cdevelopmental processes,\u201d and \u201cmetabolic processes.\u201d The present study provides a comprehensive overview of microsatellite distribution and can assist in the planning and development of potentially useful genetic tools for further investigation of <jats:italic>P. malariae<\/jats:italic> and <jats:italic>P. ovale curtisi<\/jats:italic> epidemiology.<\/jats:p>","DOI":"10.1051\/parasite\/2020034","type":"journal-article","created":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T09:09:18Z","timestamp":1589533758000},"page":"34","source":"Crossref","is-referenced-by-count":11,"title":["Genome-wide microsatellite characteristics of five human <i>Plasmodium species<\/i>, focusing on <i>Plasmodium malariae<\/i> and <i>P. ovale curtisi<\/i>"],"prefix":"10.1051","volume":"27","author":[{"given":"Vivek 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