{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:31:29Z","timestamp":1768476689878,"version":"3.49.0"},"reference-count":25,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":135,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2020,5,4]]},"published-print":{"date-parts":[[2020]]},"abstract":"<jats:p><jats:italic>Blastocystis<\/jats:italic> sp. is a ubiquitous protist that has been frequently reported in humans and animals worldwide. A total of 355 fecal samples of experimental rats were collected from four laboratory rearing facilities in China, and <jats:italic>Blastocystis<\/jats:italic> sp. was detected by PCR amplification of the partial small subunit ribosomal (SSU) rRNA gene. Twenty-nine (8.2%, 29\/355) samples were positive for <jats:italic>Blastocystis<\/jats:italic> sp., with the highest infection rate (20.7%, 24\/116) in rats of the Zhengzhou1, followed by that in the Zhengzhou2 (5.0%, 2\/40), Shenyang (3.0%, 3\/100) and Wuhan (0) rearing facilities. Among the three rat strains, Sprague\u2013Dawley (SD) rats had higher infection rates (11.3%, 17\/151) compared to Wistar rats (8.7%, 9\/104) and spontaneously hypertensive (SH) rats (3.0%, 3\/100). Two <jats:italic>Blastocystis<\/jats:italic> sp. subtypes (ST4 and ST7) were identified. ST4 was the predominant subtype detected in 26 samples (89.7%). A phylogenetic analysis demonstrated that the sequences of ST4 and ST7 obtained in this study were clustered with their reference subtypes. To our knowledge, this is the first report of <jats:italic>Blastocystis<\/jats:italic> sp. in experimental rats in China. Pathogen infections in laboratory animals need to be monitored due to fecal-oral transmission.<\/jats:p>","DOI":"10.1051\/parasite\/2020035","type":"journal-article","created":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T09:09:18Z","timestamp":1589533758000},"page":"35","source":"Crossref","is-referenced-by-count":9,"title":["Molecular identification and subtyping of <i>Blastocystis<\/i> sp. in laboratory rats in China"],"prefix":"10.1051","volume":"27","author":[{"given":"Junqiang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueyue","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuxi","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqin","family":"Chao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiqin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md Robiul","family":"Karim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Qi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"issue":"4","key":"R1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s004360050255","volume":"83","author":"Chen","year":"1997","journal-title":"Parasitology Research"},{"issue":"1","key":"R2","doi-asserted-by":"crossref","first-page":"e0169659","DOI":"10.1371\/journal.pone.0169659","volume":"12","author":"Cian","year":"2017","journal-title":"PLoS One"},{"issue":"7","key":"R3","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1007\/s10096-011-1416-6","volume":"31","author":"Forsell","year":"2012","journal-title":"European Journal of Clinical Microbiology and Infectious Diseases"},{"key":"R4","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.parint.2006.12.004","volume":"56","author":"Iguchi","year":"2007","journal-title":"Parasitology International"},{"issue":"1","key":"R5","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s00436-009-1373-5","volume":"105","author":"Iguchi","year":"2009","journal-title":"Parasitology Research"},{"issue":"9","key":"R6","doi-asserted-by":"crossref","first-page":"2841","DOI":"10.1007\/s00436-018-5973-9","volume":"117","author":"Katsumata","year":"2018","journal-title":"Parasitology Research"},{"key":"R7","unstructured":"Krinke GJ. 2000. 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