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A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detect <jats:italic>Trypanosoma brucei rhodesiense. Trypanosoma congolense<\/jats:italic> was the most prevalent <jats:italic>Trypanosoma<\/jats:italic> sp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence of <jats:italic>T. b. rhodesiense<\/jats:italic> detected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes and <jats:italic>T<\/jats:italic>. <jats:italic>b<\/jats:italic>. <jats:italic>rhodesiense<\/jats:italic> from cattle at the human\u2013livestock\u2013wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi.<\/jats:p>","DOI":"10.1051\/parasite\/2020043","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T07:50:08Z","timestamp":1595231408000},"page":"46","source":"Crossref","is-referenced-by-count":11,"title":["Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases"],"prefix":"10.1051","volume":"27","author":[{"given":"Megasari","family":"Marsela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Hayashida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisha","family":"Chatanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex Kiarie","family":"Gaithuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kawai","family":"Naoko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janelisa","family":"Musaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Yamagishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"issue":"1","key":"R1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1186\/1756-3305-6-316","volume":"6","author":"Ahmed","year":"2013","journal-title":"Parasites & Vectors"},{"issue":"6","key":"R2","first-page":"108","volume":"7","author":"Alibu","year":"2015","journal-title":"Journal of Parasitology and Vector Biology"},{"issue":"3","key":"R3","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"Altschul","year":"1990","journal-title":"Journal of Molecular Biology"},{"issue":"3","key":"R4","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1038\/nbt.3103","volume":"33","author":"Ashton","year":"2014","journal-title":"Nature Biotechnology"},{"issue":"1","key":"R5","doi-asserted-by":"crossref","first-page":"e1501","DOI":"10.1371\/journal.pntd.0001501","volume":"6","author":"Auty","year":"2012","journal-title":"PLoS Neglected Tropical Diseases"},{"issue":"17","key":"R6","first-page":"117","volume":"5","author":"Bekele","year":"2015","journal-title":"Journal of Natural Sciences Research"},{"issue":"9709","key":"R7","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0140-6736(09)60829-1","volume":"375","author":"Brun","year":"2010","journal-title":"Lancet"},{"issue":"10110","key":"R8","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.1016\/S0140-6736(17)31510-6","volume":"390","author":"B\u00fcscher","year":"2017","journal-title":"Lancet"},{"key":"R9","unstructured":"Chainey JE, Oldroyd H. 1980. 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