{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T16:56:56Z","timestamp":1776358616083,"version":"3.51.2"},"reference-count":40,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":320,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2020,11,1]]},"published-print":{"date-parts":[[2020]]},"abstract":"<jats:p><jats:italic>Giardia duodenalis<\/jats:italic> is an important zoonotic pathogen for both human and animal health. Although there have been reports on <jats:italic>G. duodenalis<\/jats:italic> infections in animals all over the world, information regarding the prevalence and genetic characteristics of <jats:italic>G. duodenalis<\/jats:italic> in sheep in Inner Mongolia, China, is limited. In this study, 209 sheep fecal specimens were collected in this autonomous region. We established that the prevalence of <jats:italic>G. duodenalis<\/jats:italic> was 64.11% (134\/209), as determined using nested PCR detection and sequences analysis of the small subunit ribosomal RNA (<jats:italic>SSU rRNA<\/jats:italic>) gene. Based on the beta-giardin (<jats:italic>bg<\/jats:italic>) locus, the glutamate dehydrogenase (<jats:italic>gdh<\/jats:italic>) locus, and the triose phosphate isomerase (<jats:italic>tpi<\/jats:italic>) locus to study genetic characteristics, both assemblages A (2.99%, 4\/134) and E (97.01%, 130\/134) were found. Five novel nucleotide sequence of assemblage E were detected, two at the <jats:italic>bg<\/jats:italic> locus, two at the <jats:italic>gdh<\/jats:italic> locus, and one at the <jats:italic>tpi<\/jats:italic> locus. Multilocus genotyping yielded four assemblage E and two assemblage A multilocus genotypes (MLGs), including four novel assemblage E MLGs and one novel assemblage A MLG. Results of this study indicated that <jats:italic>G. duodenalis<\/jats:italic> was highly prevalent in sheep in Inner Mongolia. This study is the first to use the multilocus genotyping approach to identify <jats:italic>G. duodenalis<\/jats:italic> in sheep from this region.<\/jats:p>","DOI":"10.1051\/parasite\/2020060","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T11:01:30Z","timestamp":1605524490000},"page":"60","source":"Crossref","is-referenced-by-count":13,"title":["Genetic characteristics of <i>Giardia duodenalis<\/i> from sheep in Inner Mongolia, 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