{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T19:13:25Z","timestamp":1776971605298,"version":"3.51.4"},"reference-count":65,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T00:00:00Z","timestamp":1614902400000},"content-version":"vor","delay-in-days":63,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2021,2,8]]},"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>Cystic echinococcosis, caused by infection with the larval stage of the cestode<jats:italic>Echinococcus granulosus<\/jats:italic>, is a chronic zoonosis. The lifecycle of the<jats:italic>E. granulosus<\/jats:italic>parasite includes three consecutive stages that require specific gene regulation or protein expression to survive environmental shifts between definitive hosts and intermediate hosts. The aim of the present study is to screen and analyze the stage differential antigens to be considered for vaccine development against<jats:italic>E. granulosus<\/jats:italic>. By using the iTRAQ (isobaric tags for relative and absolute quantification) method, the differentially expressed proteins were selected from the three consecutive developmental stages of<jats:italic>E. granulosus<\/jats:italic>: oncosphere, adult tapeworms, and protoscolex. Through a bioinformatics analysis including Clusters of Orthologous Groups (COG), Gene Ontology (GO), and pathway metabolic annotation, we identified some proteins of interest from each stage. The results showed that a large number of differentially expressed proteins (375: oncosphere vs. adult, 346: oncosphere vs. protoscolex, and 391: adult vs. protoscolex) were identified from the three main lifecycle stages. Analysis of the differential protein pathways showed that these differential proteins are mainly enriched in metabolic pathways, Huntington\u2019s diseases, Alzheimer\u2019s diseases, and ribosome metabolic pathways. Interestingly, among these differential proteins, expression levels of paramyosin, HSP60, HSP70, HSP90, cathepsin L1, cathepsin D, casein kinase, and calmodulin were significantly higher in the oncosphere than in the adult or protoscolex (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). We hope our findings will help to identify potential targets for diagnosis or for therapeutic and prophylactic intervention.<\/jats:p>","DOI":"10.1051\/parasite\/2021012","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T09:29:30Z","timestamp":1614936570000},"page":"15","source":"Crossref","is-referenced-by-count":13,"title":["iTRAQ-based comparative proteomic analysis in different developmental stages of<i>Echinococcus granulosus<\/i>"],"prefix":"10.1051","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-2389","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3751-7321","authenticated-orcid":false,"given":"Song","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3478-3712","authenticated-orcid":false,"given":"Xuhai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1645-1311","authenticated-orcid":false,"given":"Wenqiao","family":"Hui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4355-9862","authenticated-orcid":false,"given":"Bin","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"250","published-online":{"date-parts":[[2021,3,5]]},"reference":[{"issue":"12","key":"R1","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.ijpara.2006.06.013","volume":"36","author":"Allocco","year":"2006","journal-title":"International 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