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The efficacy of this novel formulation for the prevention of heartworm disease in cats was tested in two experimental studies using an induced infection model and a randomized, blinded, placebo-controlled study design, and two USA isolates of <jats:italic>Dirofilaria immitis<\/jats:italic>. In each study, 20 na\u00efve cats were each inoculated sub-cutaneously with 100 third-stage larvae of <jats:italic>D. immitis<\/jats:italic> 30 days before treatment. Following randomization to two treatment groups of ten cats, each cat was treated topically once, either with the minimum recommended dose of the novel formulation, or with an identical volume of placebo. Five months after treatment (6 months after infections), the cats were humanely euthanized for parasite recovery and count. Efficacy was calculated by comparison of the numbers of adult <jats:italic>D. immitis<\/jats:italic> recovered in the control and in the novel formulation groups. In the control groups of each study, <jats:italic>D. immitis<\/jats:italic> were recovered in seven and nine cats (respective worm counts ranges 1\u20137 and 1\u201316, respective geometric means 1.6 and 5.1). In both studies, none of the treated cats harbored any <jats:italic>D. immitis<\/jats:italic> at necropsy and the calculated efficacy of the novel formulation was 100%. There were no adverse reactions related to treatment with the novel formulation. The results of these two studies demonstrate that a topical NexGard<jats:sup>\u00ae<\/jats:sup> Combo application at the minimum label dose is well-tolerated and efficacious in preventing heartworm disease in cats.<\/jats:p>","DOI":"10.1051\/parasite\/2021026","type":"journal-article","created":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T09:59:28Z","timestamp":1617357568000},"page":"30","source":"Crossref","is-referenced-by-count":6,"title":["Efficacy of a novel topical combination of esafoxolaner, eprinomectin and praziquantel for the prevention of heartworm disease in cats"],"prefix":"10.1051","volume":"28","author":[{"given":"Christine","family":"Baker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"McCall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelmoneim","family":"Mansour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"McCall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayna","family":"Shaffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Wakeland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Frost","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4798-3246","authenticated-orcid":false,"given":"Eric","family":"Tielemans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwight","family":"Bowman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2021,4,2]]},"reference":[{"issue":"1","key":"R1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.2460\/javma.2005.227.139","volume":"227","author":"Atkins","year":"2005","journal-title":"Journal of the American Veterinary Medical Association"},{"issue":"1\u20132","key":"R2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.vetpar.2014.02.039","volume":"202","author":"Baker","year":"2014","journal-title":"Veterinary Parasitology"},{"issue":"40","key":"R3","first-page":"5","volume":"2004","author":"Berdoulay","year":"2004","journal-title":"Journal of the American Animal Hospital Association"},{"key":"R4","unstructured":"Beugnet F, Halos L, Guillot J. 2018. 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