{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T13:32:12Z","timestamp":1783344732644,"version":"3.54.6"},"reference-count":35,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":122,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2021,4,18]]},"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is an obligate intracellular protozoan parasite that causes toxoplasmosis and threatens warm-blooded animal and human health worldwide. Simple and applicable diagnostic methods are urgently needed to guide development of effective approaches for prevention of toxoplasmosis. Most molecular diagnostic tools for <jats:italic>T. gondii<\/jats:italic> infection require high technical skills, sophisticated equipment, and a controlled lab environment. In this study, we developed a loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) assay that specifically targets the 529 bp for detecting <jats:italic>T. gondii<\/jats:italic> infection. This novel portable device is universal, fast, user-friendly, and guarantees experimental sensitivity as well as low risk of aerosol contamination. Our LAMP-LFD assay has a detection limit of 1 fg of <jats:italic>T. gondii<\/jats:italic> DNA, and shows no cross-reaction with other parasitic pathogens, including <jats:italic>Cryptosporidium parvum<\/jats:italic>, <jats:italic>Leishmania donovani<\/jats:italic>, and <jats:italic>Plasmodium vivax<\/jats:italic>. We validated the developed assay by detecting <jats:italic>T. gondii<\/jats:italic> in DNA extracted from blood samples collected from 318 stray cats and dogs sampled from Deqing, Wenzhou, Yiwu, Lishui and Zhoushan cities across Zhejiang province, Eastern China. The LAMP-LFD device detected <jats:italic>T. gondii<\/jats:italic> DNA in 4.76 and 4.69% of stray cats and dogs, respectively. In conclusion, the developed LAMP-LFD assay is efficient, minimizes aerosol contamination, and is therefore suitable for detecting <jats:italic>T. gondii<\/jats:italic> across basic medical institutions and field settings.<\/jats:p>","DOI":"10.1051\/parasite\/2021039","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T08:12:12Z","timestamp":1620115932000},"page":"41","source":"Crossref","is-referenced-by-count":34,"title":["A novel loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) device for rapid detection of <i>Toxoplasma gondii<\/i> in the blood of stray cats and 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