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The aim of this study was to demonstrate proof-of-concept of a non-invasive molecular diagnosis technique for human filariasis by targeting fragments of 12S rDNA, Cox1, ITS1 and LL20-15kDa ladder antigen-gene by conventional PCR in DNA extracted from stool samples of 52 people infected with <jats:italic>Mansonella perstans<\/jats:italic> and\/or <jats:italic>Loa loa<\/jats:italic>. Of these, 10 patients were infected with soil-transmitted helminths (<jats:italic>Trichuris trichiura<\/jats:italic> and\/or <jats:italic>Ascaris lumbricoides<\/jats:italic>), and none were positive for <jats:italic>Necator americanus<\/jats:italic>. Interestingly, no filarial gene fragments were detected in the stools of any of the 52 patients. Future studies should evaluate whether a co-infection with soil-transmitted helminths causing gastrointestinal bleeding and likely allowing (micro)filaria exit into the digestive tract, may facilitate the molecular detection of filarial DNA fragments in stool samples.<\/jats:p>","DOI":"10.1051\/parasite\/2021046","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T08:08:02Z","timestamp":1622189282000},"page":"47","source":"Crossref","is-referenced-by-count":3,"title":["Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths"],"prefix":"10.1051","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8312-3353","authenticated-orcid":false,"given":"Maxime P. 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