{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:28:29Z","timestamp":1790252909373,"version":"4.1.0"},"reference-count":44,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":299,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"special fund for forestry reform and development of wolong special administrative region of sichuan province","award":["510000-02-064387"],"award-info":[{"award-number":["510000-02-064387"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2021,10,13]]},"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p><jats:italic>Blastocystis<\/jats:italic> is a prevalent eukaryotic parasite that has been identified in a wide range of hosts. Several species are considered potential sources of <jats:italic>Blastocystis<\/jats:italic> infection in humans, but little is known about the prevalence of <jats:italic>Blastocystis<\/jats:italic> in wild animals. In this study, the prevalence and subtypes of <jats:italic>Blastocystis<\/jats:italic> were investigated to assess the zoonotic potential of wild animals in Sichuan Wolong National Natural Reserve. A total of 300 fecal samples were collected from 27 wildlife species in three areas of the Reserve. The subtype (ST), genetic characteristics, and prevalence of <jats:italic>Blastocystis<\/jats:italic> were determined by PCR amplification of part (~600\u00a0bp) of the SSU rRNA gene. Thirty fecal samples (10.0%) were <jats:italic>Blastocystis<\/jats:italic>-positive. The highest prevalence of <jats:italic>Blastocystis<\/jats:italic> was found in Yinchanggou (18.3%), with significantly less found in Niutoushan (7.5%) and Genda (5.5%) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). No significant differences were associated with different orders of animals in prevalence, which may be because of the small number of positive samples obtained. Sequence analysis showed five subtypes (ST1, ST3, ST5, ST13, and ST14), with ST13 and ST14 being predominant (33% each), followed by ST1 (20%). This is the first molecular investigation of <jats:italic>Blastocystis<\/jats:italic> infection in the wild animals of southwestern China. Subtypes ST1, ST3, ST5, and ST14 have previously been identified in humans, suggesting that wild animals may be potential reservoirs of <jats:italic>Blastocystis<\/jats:italic> for humans.<\/jats:p>","DOI":"10.1051\/parasite\/2021071","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T18:58:11Z","timestamp":1635361091000},"page":"73","source":"Crossref","is-referenced-by-count":13,"title":["Genetic characterization and zoonotic potential of <i>Blastocystis<\/i> from wild animals in Sichuan Wolong National Natural Reserve, Southwest China"],"prefix":"10.1051","volume":"28","author":[{"given":"Shanyu","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanyu","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaogang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Chai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehong","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuhong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingmei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangneng","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"250","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"issue":"12","key":"R1","doi-asserted-by":"crossref","first-page":"e84372","DOI":"10.1371\/journal.pone.0084372","volume":"8","author":"Abdulsalam","year":"2013","journal-title":"PLoS One"},{"issue":"3","key":"R2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0304-4017(02)00050-X","volume":"106","author":"Abe","year":"2002","journal-title":"Veterinary Parasitology"},{"issue":"1","key":"R3","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.actatropica.2012.12.011","volume":"126","author":"Alfellani","year":"2013","journal-title":"Acta Tropica"},{"issue":"4","key":"R4","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.protis.2013.05.003","volume":"164","author":"Alfellani","year":"2013","journal-title":"Protist"},{"issue":"3","key":"R5","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1128\/JCM.02520-15","volume":"54","author":"Andersen","year":"2016","journal-title":"Journal of Clinical Microbiology"},{"issue":"12","key":"R6","doi-asserted-by":"crossref","first-page":"2848","DOI":"10.1038\/ismej.2017.139","volume":"11","author":"Beghini","year":"2017","journal-title":"ISME Journal"},{"issue":"9","key":"R7","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1017\/S0031182017002347","volume":"145","author":"Betts","year":"2018","journal-title":"Parasitology"},{"key":"R8","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3389\/fmicb.2020.00288","volume":"11","author":"Betts","year":"2020","journal-title":"Frontiers in Microbiology"},{"issue":"1","key":"R9","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1756-3305-1-40","volume":"1","author":"Boorom","year":"2008","journal-title":"Parasites & Vectors"},{"key":"R10","first-page":"143","volume":"11","author":"Chai","year":"2020","journal-title":"International Journal for Parasitology: Parasites and Wildlife"},{"key":"R11","doi-asserted-by":"crossref","unstructured":"Chen S, Meng W, Zhou Z, Deng L, Shi X, Chai Y, Liu H, Cheng Y, Zhong Z, Fu H, Shen L, Zhang K, He T, Peng G. 2021. 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