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Current treatments have numerous side effects and chemoresistance is emerging, hence the need to find new anti-<jats:italic>Toxoplasma gondii<\/jats:italic> substances. This study focuses on the antiparasitic potential of lupane-type pentacyclic triterpenes isolated from the bark of black alder (<jats:italic>Alnus glutinosa<\/jats:italic>), as well as the hypothesis of their macromolecular target by an original method of reverse docking. Among the isolated triterpenes, betulone was the most active compound with an IC<jats:sub>50<\/jats:sub> of 2.7\u00a0\u00b1\u00a01.2\u00a0\u03bcM, a CC<jats:sub>50<\/jats:sub> greater than 80\u00a0\u03bcM, and a selectivity index of over 29.6. An additional study of the anti-<jats:italic>T. gondii<\/jats:italic> potential of commercially available compounds (betulonic acid methyl ester and betulonic acid) showed the important role of the C3 ketone function and the C28 oxidation level on the lupane-type triterpene in the antiparasitic activity since their IC<jats:sub>50<\/jats:sub> and CC<jats:sub>50<\/jats:sub> were similar to that of betulone. Finally, the most active compounds were subjected to the AMIDE reverse docking workflow. A dataset of 87 <jats:italic>T. gondii<\/jats:italic> proteins from the Protein Data Bank was created. It identified calcium-dependent protein kinase CDPK3 as the most likely target of betulin derivatives.<\/jats:p>","DOI":"10.1051\/parasite\/2022008","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T09:02:11Z","timestamp":1644483731000},"page":"7","source":"Crossref","is-referenced-by-count":18,"title":["Anti-<i>Toxoplasma gondii<\/i> effect of lupane-type triterpenes from the bark of black alder (<i>Alnus glutinosa<\/i>) and identification of a potential target by reverse 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