{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T21:12:41Z","timestamp":1772658761054,"version":"3.50.1"},"reference-count":40,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":80,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["No.2017YFD0501202"],"award-info":[{"award-number":["No.2017YFD0501202"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"International Science & Technology Cooperation Program of XPCC","award":["No. 2016AH006"],"award-info":[{"award-number":["No. 2016AH006"]}]},{"name":"Xinjiang Autonomous Region graduate innovation project","award":["No. XJ2020G085"],"award-info":[{"award-number":["No. XJ2020G085"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2022,2,28]]},"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>Fascioliasis is an important zoonotic helminthic disease caused by<jats:italic>Fasciola hepatica<\/jats:italic>and poses a serious threat to global public health. To evade the immune response of its host (humans or animals),<jats:italic>F. hepatica<\/jats:italic>secretes various antioxidant enzymes such as glutathione transferase (GST) to facilitate its invasion, migration and parasitism<jats:italic>in vivo<\/jats:italic>. To investigate the biological functions of a novel omega-class GST (GSTO), the molecular features of GSTO2 of<jats:italic>F. hepatica<\/jats:italic>were analyzed by online software, and the biochemical properties<jats:italic>in vitro<\/jats:italic>of recombinant GSTO2 (rGSTO2) were dissected. Then, the regulatory roles of rGSTO2 protein in murine macrophages<jats:italic>in vitro<\/jats:italic>were further explored. The results revealed that the<jats:italic>GSTO2<\/jats:italic>gene encodes 254 amino acids, which harbor the characteristic N-terminal domain (\u03b2\u03b1\u03b2\u03b1\u03b2\u03b2\u03b1) and C-terminal domain (\u03b1-helical) of the cytoplasmic GST superfamily. GSTO2 was mainly expressed in<jats:italic>F. hepatica<\/jats:italic>vitelline follicles, intestinal tract, excretory pores and vitelline cells, with thioltransferase and dehydroascorbate reductase activities. Moreover, rGSTO2 protein could be taken up by murine macrophages and significantly inhibit the viability of macrophages. In addition, rGSTO2 protein could significantly promote apoptosis and modulate the expression of cytokines in macrophages. These findings suggested that<jats:italic>F. hepatica<\/jats:italic>GSTO2 plays an important role in modulating the physiological functions of macrophages, whereby this protein might be involved in immunomodulatory and anti-inflammatory roles during infection. This study provided new insights into the immune-evasion mechanism of<jats:italic>F. hepatica<\/jats:italic>and may contribute to the development of a potential anti-inflammatory agent.<\/jats:p>","DOI":"10.1051\/parasite\/2022016","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T08:57:26Z","timestamp":1647939446000},"page":"16","source":"Crossref","is-referenced-by-count":10,"title":["Molecular characterization of a novel GSTO2 of<i>Fasciola hepatica<\/i>and its roles in modulating murine 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