{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T11:58:13Z","timestamp":1791374293696,"version":"4.3.1"},"reference-count":33,"publisher":"EDP Sciences","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"vor","delay-in-days":87,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite"],"accepted":{"date-parts":[[2022,3,11]]},"published-print":{"date-parts":[[2022]]},"abstract":"<jats:p>Artesunate is the current most potent antimalarial drug widely used for the treatment of malaria. Considering the emergence of artemisinin resistance, several situations may require a simple method for artesunate quantification. We thus developed a quantitative and a semi-quantitative biological method for the determination of artesunate in liquid samples. The tests are based on the measurement of samples\u2019 antimalarial activity on<jats:italic>Plasmodium falciparum<\/jats:italic>3D7 using a modified SYBR Green I drug susceptibility test. For the quantitative test, we established a standard curve that resulted from a dose\u2013response curve and evaluated its performances using controls samples. Whereas the linear regression analysis between artesunate concentration and antimalarial activity showed promising results (linearity range 1.5\u201324.6 ng\/mL,<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.9373), we found that artesunate content of the controls was significantly overestimated (<jats:italic>p<\/jats:italic>= 0.0313). For the semi-quantitative test, we compared the antimalarial activities of samples collected during permeation studies of artesunate to that of a reference (artesunate IC<jats:sub>50<\/jats:sub>) by statistical analysis. We demonstrated that antimalarial activities of samples from permeation tests using a powder formulation of artesunate were greater than those of samples from tests using a solution formulation. Bioassays can be simple techniques to assess artesunate in liquid samples, particularly in resource-limited settings. Comparison with reference methods is still recommended when accurate drug quantification is required.<\/jats:p>","DOI":"10.1051\/parasite\/2022019","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T07:55:21Z","timestamp":1648540521000},"page":"18","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of quantitative and semi-quantitative biological test methods of artesunate<i>in vitro<\/i>"],"prefix":"10.1051","volume":"29","author":[{"given":"Yobouet Ines","family":"Kouakou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roukayatou","family":"Omorou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim 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